Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1

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dc.contributor.author Mahony, Jennifer
dc.contributor.author Alqarni, Mona
dc.contributor.author Stockdale, Stephen
dc.contributor.author Spinelli, Silvia
dc.contributor.author Feyereisen, Marine
dc.contributor.author Cambillau, Christian
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2016-12-02T09:43:15Z
dc.date.available 2016-12-02T09:43:15Z
dc.date.issued 2016-11-08
dc.identifier.citation Mahony, J., Alqarni, M., Stockdale, S., Spinelli, S., Feyereisen, M., Cambillau, C. and van Sinderen, D. (2016) 'Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1', Scientific Reports, 6, pp. 1-10. doi:10.1038/srep36667 en
dc.identifier.volume 6 en
dc.identifier.startpage 1 en
dc.identifier.endpage 10 en
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10468/3342
dc.identifier.doi 10.1038/srep36667
dc.description.abstract The tail tape measure protein (TMP) of tailed bacteriophages (also called phages) dictates the tail length and facilitates DNA transit to the cell cytoplasm during infection. Here, a thorough mutational analysis of the TMP from lactococcal phage TP901-1 (TMPTP901-1) was undertaken. We generated 56 mutants aimed at defining TMPTP901-1 domains that are essential for tail assembly and successful infection. Through analysis of the derived mutants, we determined that TP901-1 infectivity requires the N-terminal 154 aa residues, the C-terminal 60 residues and the first predicted hydrophobic region of TMPTP901-1 as a minimum. Furthermore, the role of TMPTP901-1 in tail length determination was visualized by electron microscopic imaging of TMP-deletion mutants. The inverse linear correlation between the extent of TMPTP901-1-encoding gene deletions and tail length of the corresponding virion provides an estimate of TMPTP901-1 regions interacting with the connector or involved in initiator complex formation. This study represents the most thorough characterisation of a TMP from a Gram-positive host-infecting phage and provides essential advances to understanding its role in virion assembly, morphology and infection. en
dc.description.sponsorship Science Foundation Ireland (Technology Innovation Development Award (TIDA) Ref. No. 14/TIDA/2287; Principal Investigator Award Ref. No. 13/IA/1953); Saudi Arabian Cultural Bureau (King Abdullah Scholarship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Springer Nature en
dc.rights © 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Bacteriophage lambda tail en
dc.subject Length determination en
dc.subject Membrane protein en
dc.subject Infection en
dc.subject Baseplate en
dc.subject Receptor en
dc.subject Host en
dc.subject Chaperone en
dc.subject Lactis en
dc.subject Genes en
dc.title Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2016-12-02T09:28:48Z
dc.description.version Published Version en
dc.internal.rssid 374128627
dc.internal.wokid WOS:000387323800001
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Saudi Arabian Cultural Bureau
dc.description.status Peer reviewed en
dc.identifier.journaltitle Scientific Reports en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid 36667


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© 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as © 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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