The exploration of potato-associated bacteria in the Central Andean Highlands and their application in integrated crop management systems

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dc.contributor.advisor Doyle Prestwich, Barbara en
dc.contributor.author Velivelli, Siva L. S.
dc.date.accessioned 2016-12-08T09:53:29Z
dc.date.issued 2014
dc.date.submitted 2014
dc.identifier.citation Velivelli, S. L. S. 2014. The exploration of potato-associated bacteria in the Central Andean Highlands and their application in integrated crop management systems. PhD Thesis, University College Cork. en
dc.identifier.endpage 208 en
dc.identifier.uri http://hdl.handle.net/10468/3357
dc.description.abstract Potato is the most important food crop after wheat and rice. A changing climate, coupled with a heightened consumer awareness of how food is produced and legislative changes governing the usage of agrochemicals, means that alternative more integrated and sustainable approaches are needed for crop management practices. Bioprospecting in the Central Andean Highlands resulted in the isolation and in vitro screening of 600 bacterial isolates. The best performing isolates, under in vitro conditions, were field trialled in their home countries. Six of the isolates, Pseudomonas sp. R41805 (Bolivia), Pseudomonas palleroniana R43631 (Peru), Bacillus sp. R47065, R47131, Paenibacillus sp. B3a R49541, and Bacillus simplex M3-4 R49538 (Ecuador), showed significant increase in the yield of potato. Using – omic technologies (i.e. volatilomic, transcriptomic, proteomic and metabolomic), the influence of microbial isolates on plant defence responses was determined. Volatile organic compounds of bacterial isolates were identified using GC/MS. RT-qPCR analysis revealed the significant expression of Ethylene Response Factor 3 (ERF3) and the results of this study suggest that the dual inoculation of potato with Pseudomonas sp. R41805 and Rhizophagus irregularis MUCL 41833 may play a part in the activation of plant defence system via ERF3. The proteomic analysis by 2-DE study has shown that priming by Pseudomonas sp. R41805 can induce the expression of proteins related to photosynthesis and protein folding in in vitro potato plantlets. The metabolomics study has shown that the total glycoalkaloid (TGA) content of greenhouse-grown potato tubers following inoculation with Pseudomonas sp. R41805 did not exceed the acceptable safety limit (200 mg kg-1 FW). As a result of this study, a number of bacteria have been identified with commercial potential that may offer sustainable alternatives in both Andean and European agricultural settings. en
dc.description.sponsorship Seventh Framework Programme (EU-FP7 grant agreement No 227522) en
dc.format.mimetype application/pdf en
dc.language English en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2014, Siva L.S. Velivelli. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Rhizoctonia solani en
dc.subject Phytophthora infestans en
dc.subject Potato en
dc.subject Arbuscular mycorrhizal fungi en
dc.subject Plant growth-promoting rhizobacteria en
dc.subject Volatile organic compounds en
dc.subject Gas chromatography/mass spectrometry en
dc.subject Ethylene response factor 3 en
dc.subject Sustainable agriculture en
dc.subject Biological control en
dc.subject Two-dimensional gel electrophoresis en
dc.subject Defence gene expression en
dc.title The exploration of potato-associated bacteria in the Central Andean Highlands and their application in integrated crop management systems en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text not available en
dc.check.info Indefinite en
dc.check.date 10000-01-01
dc.description.version Accepted Version
dc.contributor.funder Seventh Framework Programme en
dc.description.status Not peer reviewed en
dc.internal.school Biological, Earth & Environmental Sciences en
dc.check.reason This thesis contains third party copyrighted materials for which permission was not given for online use en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.entireThesis Entire Thesis Restricted
dc.check.embargoformat Both hard copy thesis and e-thesis en
ucc.workflow.supervisor b.doyle@ucc.ie
dc.internal.conferring Spring 2015 en


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© 2014, Siva L.S. Velivelli. Except where otherwise noted, this item's license is described as © 2014, Siva L.S. Velivelli.
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