Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways
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Date
2016-12-08
Authors
James, Kieran
O'Connell Motherway, Mary
Bottacini, Francesca
van Sinderen, Douwe
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Publisher
Nature Publishing Group
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Abstract
In this study, we demonstrate that the prototype B. breve strain UCC2003 possesses specific metabolic pathways for the utilisation of lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT), which represent the central moieties of Type I and Type II human milk oligosaccharides (HMOs), respectively. Using a combination of experimental approaches, the enzymatic machinery involved in the metabolism of LNT and LNnT was identified and characterised. Homologs of the key genetic loci involved in the utilisation of these HMO substrates were identified in B. breve, B. bifidum, B. longum subsp. infantis and B. longum subsp. longum using bioinformatic analyses, and were shown to be variably present among other members of the Bifidobacterium genus, with a distinct pattern of conservation among human-associated bifidobacterial species.
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Keywords
Longum subsp. infantis , DNA-microarray data , Lactococcus lactis , Gut microbiota , Molecular cloning , Gene expression , Necrotizing enterocolitis , Beta-galactosidases , Escherichia-coli , Bifidum
Citation
James, K., O’Connell Motherway, M., Bottacini, F. and van Sinderen, D. (2016) ‘Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways’, Scientific Reports, 6, 38560 (16pp). doi: 10.1038/srep38560
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© 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/