Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways

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dc.contributor.author James, Kieran
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Bottacini, Francesca
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2017-01-09T10:48:25Z
dc.date.available 2017-01-09T10:48:25Z
dc.date.issued 2016-12-08
dc.identifier.citation James, K., O’Connell Motherway, M., Bottacini, F. and van Sinderen, D. (2016) ‘Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways’, Scientific Reports, 6, 38560 (16pp). doi: 10.1038/srep38560 en
dc.identifier.volume 6 en
dc.identifier.startpage 1 en
dc.identifier.endpage 16 en
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10468/3448
dc.identifier.doi 10.1038/srep38560
dc.description.abstract In this study, we demonstrate that the prototype B. breve strain UCC2003 possesses specific metabolic pathways for the utilisation of lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT), which represent the central moieties of Type I and Type II human milk oligosaccharides (HMOs), respectively. Using a combination of experimental approaches, the enzymatic machinery involved in the metabolism of LNT and LNnT was identified and characterised. Homologs of the key genetic loci involved in the utilisation of these HMO substrates were identified in B. breve, B. bifidum, B. longum subsp. infantis and B. longum subsp. longum using bioinformatic analyses, and were shown to be variably present among other members of the Bifidobacterium genus, with a distinct pattern of conservation among human-associated bifidobacterial species. en
dc.description.sponsorship Irish Research Council (Postgraduate Research Project Award: Project ID GOIPG/2013/651); Science Foundation Ireland (Grant No. SFI/12/RC/2273); Health Research Board (Postdoctoral Fellowship Grant No. PDTM/20011/9) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Nature Publishing Group en
dc.rights © 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ en
dc.subject Longum subsp. infantis en
dc.subject DNA-microarray data en
dc.subject Lactococcus lactis en
dc.subject Gut microbiota en
dc.subject Molecular cloning en
dc.subject Gene expression en
dc.subject Necrotizing enterocolitis en
dc.subject Beta-galactosidases en
dc.subject Escherichia-coli en
dc.subject Bifidum en
dc.title Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-01-09T10:35:08Z
dc.description.version Published Version en
dc.internal.rssid 378964292
dc.internal.wokid WOS:000389663200001
dc.contributor.funder Irish Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Health Research Board en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Scientific Reports en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.internal.IRISemailaddress kieran.james@ucc.ie en
dc.identifier.articleid 38560


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