Harnessing bacterial signals for suppression of biofilm formation in the nosocomial fungal pathogen Aspergillus fumigatus

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dc.contributor.author Reen, F. Jerry
dc.contributor.author Phelan, John P.
dc.contributor.author Woods, David F.
dc.contributor.author Shanahan, Rachel
dc.contributor.author Cano, Rafael
dc.contributor.author Clarke, Sarah L.
dc.contributor.author McGlacken, Gerard P.
dc.contributor.author O'Gara, Fergal
dc.date.accessioned 2017-01-09T14:59:01Z
dc.date.available 2017-01-09T14:59:01Z
dc.date.issued 2016-12-22
dc.identifier.citation Reen, F. J., Phelan, J. P., Woods, D. F., Shanahan, R., Cano, R., Clarke, S., McGlacken, G. P. and O’Gara, F. (2016) 'Harnessing Bacterial Signals for Suppression of Biofilm Formation in the Nosocomial Fungal Pathogen Aspergillus fumigatus', Frontiers in Microbiology, 7(2074). doi:10.3389/fmicb.2016.02074 en
dc.identifier.volume 7 en
dc.identifier.startpage 2074-1 en
dc.identifier.endpage 2074-12 en
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/3451
dc.identifier.doi 10.3389/fmicb.2016.02074
dc.description.abstract Faced with the continued emergence of antibiotic resistance to all known classes of antibiotics, a paradigm shift in approaches toward antifungal therapeutics is required. Well characterized in a broad spectrum of bacterial and fungal pathogens, biofilms are a key factor in limiting the effectiveness of conventional antibiotics. Therefore, therapeutics such as small molecules that prevent or disrupt biofilm formation would render pathogens susceptible to clearance by existing drugs. This is the first report describing the effect of the Pseudomonas aeruginosa alkylhydroxyquinolone interkingdom signal molecules 2-heptyl-3-hydroxy-4-quinolone and 2-heptyl-4-quinolone on biofilm formation in the important fungal pathogen Aspergillus fumigatus. Decoration of the anthranilate ring on the quinolone framework resulted in significant changes in the capacity of these chemical messages to suppress biofilm formation. Addition of methoxy or methyl groups at the C5–C7 positions led to retention of anti-biofilm activity, in some cases dependent on the alkyl chain length at position C2. In contrast, halogenation at either the C3 or C6 positions led to loss of activity, with one notable exception. Microscopic staining provided key insights into the structural impact of the parent and modified molecules, identifying lead compounds for further development. en
dc.description.sponsorship European Commission (FP7-PEOPLE-2013-ITN, 607786; FP7-KBBE-2012-6, CP-TP-312184; FP7-KBBE-2012-6, 311975; OCEAN 2011-2, 287589; Marie Curie 256596; EU-634486); Science Foundation Ireland ( SFI SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438, grant number SFI/12/RC/2275); Marine Institute (Beaufort award C2CRA 2007/082); Department of Agriculture and Food, Ireland (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council for Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647); Health Research Board/Irish Thoracic Society (MRCG-2014-6); Teagasc (Walsh Fellowship 2013) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.rights © 2016 Reen, Phelan, Woods, Shanahan, Cano, Clarke, McGlacken and O’Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Biofilm en
dc.subject Aspergillus fumigatus en
dc.subject Pseudomonas aeruginosa en
dc.subject Interkingdom Interactions en
dc.subject Alkylhydroxyquinolone (AHQ) en
dc.subject PQS en
dc.subject HHQ en
dc.title Harnessing bacterial signals for suppression of biofilm formation in the nosocomial fungal pathogen Aspergillus fumigatus en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Fergal O'Gara, Microbiology , University College Cork, Cork, Ireland. +353-21-490-3000 Email: f.ogara@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-01-09T14:35:14Z
dc.description.version Published Version en
dc.internal.rssid 378964318
dc.contributor.funder European Commission en
dc.contributor.funder Seventh Framework Programme en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Marine Institute en
dc.contributor.funder Irish Research Council for Science, Engineering and Technology en
dc.contributor.funder Health Research Board en
dc.contributor.funder Teagasc en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers In Microbiology en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress f.ogara@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3 en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATION en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE en


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© 2016 Reen, Phelan, Woods, Shanahan, Cano, Clarke, McGlacken and O’Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2016 Reen, Phelan, Woods, Shanahan, Cano, Clarke, McGlacken and O’Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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