Microbially derived bioactive peptides to improve human health

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dc.contributor.advisor Ross, R. Paul en
dc.contributor.advisor Fitzgerald, Gerald F. en
dc.contributor.advisor Stanton, Catherine en
dc.contributor.author Kent, Robert M.
dc.date.accessioned 2017-01-11T10:13:58Z
dc.date.issued 2014
dc.date.submitted 2014
dc.identifier.citation Kent, R .M. 2014. Microbially derived bioactive peptides to improve human health. PhD Thesis, University College Cork. en
dc.identifier.endpage 222 en
dc.identifier.uri http://hdl.handle.net/10468/3463
dc.description.abstract This thesis describes a study of various methods to produce bioactive peptides. Initially, the generation of anti-Cronobacter spp. peptides by fermentation of milk protein is described. Lactobacillus johnsonii DPC6026 was used to generate two previously described antimicrobial peptides. Phenotypic analysis indicated unsatisfactory casein hydrolysis. The genome of the strain was sequenced and annotated. Results showed a number of unique features present, most notably a large symmetrical inversion of approximately 750kb in comparison with the human isolate L. johnsonii NCC 533. The data suggest significant genetic diversity and intra-species genomic rearrangements within the L. johnsonii spp.. Cronobacter spp. have emerged as pathogens of concern to the powdered infant formula industry. Chapters 3 and 4 of this thesis describe novel methods to generate two antimicrobial peptides, Caseicin A and B. In Chapter 3 a bank of Bacillus strains was generated and investigated for caseicin production. Following casein hydrolysis by specific B. cereus and B. thuringiensis strains the peptides of interest were generated. Chapter 4 describes a sterile enzymatic method to generate peptides from casein. Bioinformatic tools were used to predict enzymes capable of liberating caseicin peptides from casein. Hydrolysates were generated using suitable enzymes, examined and some were found to produce peptides with activity against Cronobacter spp.. This study establishes a potential industrial-grade method to generate antimicrobial peptides. Administration of GLP-1 leads to improved glycaemic control in diabetes patients. Generation of a recombinant lactic acid bacteria capable of producing a GLP-1 analogue is described in Chapter 5. In-vivo analysis confirmed insulinotropic activity. The results illustrate a method using bacteriocin producing cellular machinery to generate bioactive peptides. This thesis describes the generation of bioactive peptides by bacterial fermentation, tailored enzymatic hydrolysis and recombinant bacterial methods. The techniques described contribute to bioactive peptide research with regards novel methods of production and industrial scale-up. en
dc.description.sponsorship Enterprise Ireland (Grant CFTD/07/116) en
dc.format.mimetype application/pdf en
dc.language English en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2014, Robert Martin Kent. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Bioactive peptides en
dc.subject Antimicrobial en
dc.subject Cronobacter en
dc.subject GLP-1 en
dc.subject Diabetes en
dc.title Microbially derived bioactive peptides to improve human health en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en
dc.description.version Accepted Version
dc.contributor.funder Enterprise Ireland en
dc.description.status Not peer reviewed en
dc.internal.school Microbiology en
dc.internal.school Teagasc en
dc.check.reason This thesis is due for publication or the author is actively seeking to publish this material en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.chapterOfThesis 5
dc.check.embargoformat E-thesis on CORA only en
ucc.workflow.supervisor g.fitzgerald@ucc.ie
dc.internal.conferring Summer 2015 en

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