The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

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dc.contributor.author Clamens, Thomas
dc.contributor.author Rosay, Thibaut
dc.contributor.author Crépin, Alexandre
dc.contributor.author Grandjean, Teddy
dc.contributor.author Kentache, Takfarinas
dc.contributor.author Hardouin, Julie
dc.contributor.author Bortolotti, Perrine
dc.contributor.author Neidig, Anke
dc.contributor.author Mooij, Marlies J.
dc.contributor.author Hillion, Mélanie
dc.contributor.author Vieillard, Julien
dc.contributor.author Cosette, Pascal
dc.contributor.author Overhage, Joerg
dc.contributor.author O'Gara, Fergal
dc.contributor.author Bouffartigues, Emeline
dc.contributor.author Dufour, Alain
dc.contributor.author Chevalier, Sylvie
dc.contributor.author Guery, Benoit
dc.contributor.author Cornelis, Pierre
dc.contributor.author Feuilloley, Marc G. J.
dc.contributor.author Lesouhaitier, Olivier
dc.date.accessioned 2017-02-03T09:56:54Z
dc.date.available 2017-02-03T09:56:54Z
dc.date.issued 2017-01-24
dc.identifier.citation Clamens, T., Rosay, T., Crépin, A., Grandjean, T., Kentache, T., Hardouin, J., Bortolotti, P., Neidig, A., Mooij, M., Hillion, M., Vieillard, J., Cosette, P., Overhage, J., O’Gara, F., Bouffartigues, E., Dufour, A., Chevalier, S., Guery, B., Cornelis, P., Feuilloley, M. G. J. and Lesouhaitier, O. (2017) 'The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence', Scientific Reports, 7, pp. 41178. doi:10.1038/srep41178 en
dc.identifier.volume 7 en
dc.identifier.startpage 41178-1 en
dc.identifier.endpage 41178-16 en
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10468/3559
dc.identifier.doi 10.1038/srep41178
dc.description.abstract We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE+) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE+ strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication. en
dc.description.sponsorship Ministère des Affaires Étrangères (Partenariats Hubert Curien (PHC Ulysses)); Karlsruher Institut für Technologie (BioInterfaces (BIF) Program of the Karlsruhe Institute of Technology (KIT) in the Helmholtz Association)
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Nature Publishing Group en
dc.rights © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Bacteriology en
dc.subject Microbiology en
dc.subject Pseudomonas aeruginosa virulence en
dc.subject Virulence regulation en
dc.subject AmiE overproduction en
dc.title The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Fergal O'Gara, Microbiology , University College Cork, Cork, Ireland. +353-21-490-3000 Email: f.ogara@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-02-03T09:41:44Z
dc.description.version Published Version en
dc.internal.rssid 382146089
dc.contributor.funder Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
dc.contributor.funder European Regional Development Fund
dc.contributor.funder Association Vaincre la Mucoviscidose
dc.contributor.funder Ministère des Affaires Étrangères
dc.contributor.funder Karlsruher Institut für Technologie
dc.description.status Peer reviewed en
dc.identifier.journaltitle Scientific Reports en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress f.ogara@ucc.ie en


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© The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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