Antibiotic susceptibility profiles of dairy Leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix

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dc.contributor.author Flórez, Ana Belén
dc.contributor.author Campedelli, Ilenia
dc.contributor.author Delgado, Susana
dc.contributor.author Alegría, Ángel
dc.contributor.author Salvetti, Elisa
dc.contributor.author Felis, Giovanna E.
dc.contributor.author Mayo, Baltasar
dc.contributor.author Torriani, Sandra
dc.date.accessioned 2017-02-27T12:45:52Z
dc.date.available 2017-02-27T12:45:52Z
dc.date.issued 2016-01-04
dc.identifier.citation Flórez, A. B., Campedelli, I., Delgado, S., Alegría, Á., Salvetti, E., Felis, G. E., Mayo, B. and Torriani, S. (2016) 'Antibiotic Susceptibility Profiles of Dairy Leuconostoc, Analysis of the Genetic Basis of Atypical Resistances and Transfer of Genes In Vitro and in a Food Matrix', PLOS ONE, 11(1), pp. e0145203. doi:10.1371/journal.pone.0145203 en
dc.identifier.volume 11 en
dc.identifier.startpage e0145203-1 en
dc.identifier.endpage e0145203-20 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/3697
dc.identifier.doi 10.1371/journal.pone.0145203
dc.description.abstract In spite of a global concern on the transfer of antibiotic resistances (AR) via the food chain, limited information exists on this issue in species of Leuconostoc and Weissella, adjunct cultures used as aroma producers in fermented foods. In this work, the minimum inhibitory concentration was determined for 16 antibiotics in 34 strains of dairy origin, belonging to Leuconostoc mesenteroides (18), Leuconostoc citreum (11), Leuconostoc lactis (2), Weissella hellenica (2), and Leuconostoc carnosum (1). Atypical resistances were found for kanamycin (17 strains), tetracycline and chloramphenicol (two strains each), and erythromycin, clindamycin, virginiamycin, ciprofloxacin, and rifampicin (one strain each). Surprisingly, L. mesenteroides subsp. mesenteroides LbE16, showed resistance to four antibiotics, kanamycin, streptomycin, tetracycline and virginiamycin. PCR analysis identified tet(S) as responsible for tetracycline resistance in LbE16, but no gene was detected in a second tetracycline-resistant strain, L. mesenteroides subsp. cremoris LbT16. In Leuconostoc mesenteroides subsp. dextranicum LbE15, erythromycin and clindamycin resistant, an erm(B) gene was amplified. Hybridization experiments proved erm(B) and tet(S) to be associated to a plasmid of ≈35 kbp and to the chromosome of LbE15 and LbE16, respectively. The complete genome sequence of LbE15 and LbE16 was used to get further insights on the makeup and genetic organization of AR genes. Genome analysis confirmed the presence and location of erm(B) and tet(S), but genes providing tetracycline resistance in LbT16 were again not identified. In the genome of the multi-resistant strain LbE16, genes that might be involved in aminoglycoside (aadE, aphA-3, sat4) and virginiamycin [vat(E)] resistance were further found. The erm(B) gene but not tet(S) was transferred from Leuconostoc to Enterococcus faecalis both under laboratory conditions and in cheese. This study contributes to the characterization of AR in the Leuconostoc-Weissella group, provides evidence of the genetic basis of atypical resistances, and demonstrates the inter-species transfer of erythromycin resistance. en
dc.description.sponsorship Spain-Italy bilateral collaboration program (Ref. Ministerio de Educatiòn y Ciencia IT2009-0080, and Ministerio dell'Istruzione, dell'Univesità e della Ricerca IT105MD12L en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2016 Flórez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Antibiotic resistance en
dc.subject Antibiotics en
dc.subject Antimicrobial resistance en
dc.subject Enterococcus faecalis en
dc.subject Polymerase chain reaction en
dc.subject Erythromycin en
dc.subject Tetracyclines en
dc.subject Chloramphenicol en
dc.title Antibiotic susceptibility profiles of dairy Leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Elisa Salvetti, APC Microbiome Institute, University College Cork, Cork, Ireland T: +353 21 490 3000 E: elisa.salvetti@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.copyrightchecked !!CORA!! en
dc.internal.IRISemailaddress elisa.salvetti@ucc.ie en


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© 2016 Flórez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2016 Flórez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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