Whole-metagenome-sequencing-based community profiles of Vitis vinifera L. cv. Corvina berries withered in two post-harvest conditions

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dc.contributor.author Salvetti, Elisa
dc.contributor.author Campanaro, Stefano
dc.contributor.author Campedelli, Ilenia
dc.contributor.author Fracchetti, Fabio
dc.contributor.author Gobbi, Alex
dc.contributor.author Tornielli, Giovanni Battista
dc.contributor.author Torriani, Sandra
dc.contributor.author Felis, Giovanna E.
dc.date.accessioned 2017-02-27T14:45:48Z
dc.date.available 2017-02-27T14:45:48Z
dc.date.issued 2016-06-23
dc.identifier.citation Salvetti, E., Campanaro, S., Campedelli, I., Fracchetti, F., Gobbi, A., Tornielli, G. B., Torriani, S. and Felis, G. E. (2016) 'Whole-Metagenome-Sequencing-Based Community Profiles of Vitis vinifera L. cv. Corvina Berries Withered in Two Post-harvest Conditions', Frontiers in Microbiology, 7(937). doi:10.3389/fmicb.2016.00937 en
dc.identifier.volume 7 en
dc.identifier.startpage 937-1 en
dc.identifier.endpage 937-17 en
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/3698
dc.identifier.doi 10.3389/fmicb.2016.00937
dc.description.abstract Vitis vinifera L. cv. Corvina grape forms the basis for the production of unique wines, such as Amarone, whose distinctive sensory features are strongly linked to the post-harvest grape withering process. Indeed, this process increases sugar concentration and changes must characteristics. While microorganisms involved in must fermentation have been widely investigated, few data are available on the microbiota of withered grapes. Thus, in this paper, a whole metagenome sequencing (WMS) approach was used to analyse the microbial consortium associated with Corvina berries at the end of the withering process performed in two different conditions (“traditional withering,” TW or “accelerated withering,” AW), and to unveil whether changes of drying parameters could have an impact on microbial diversity. Samples of healthy undamaged berries were collected and washed, to recover microorganisms from the surface and avoid contamination with grapevine genetic material. Isolated DNA was sequenced and the data obtained were analyzed with several bioinformatics methods. The eukaryotic community was mainly composed by members of the phylum Ascomycota, including Eurotiomycetes, Sordariomycetes, and Dothideomycetes. Moreover, the distribution of the genera Aspergillus and Penicillium (class Eurotiomycetes) varied between the withered berry samples. Instead, Botryotinia, Saccharomyces, and other wine technologically useful microorganisms were relatively scarce in both samples. For prokaryotes, 25 phyla were identified, nine of which were common to both conditions. Environmental bacteria belonging to the class Gammaproteobacteria were dominant and, in particular, the TW sample was characterized by members of the family Pseudomonadaceae, while members of the family Enterobacteriaceae dominated the AW sample, in addition to Sphyngobacteria and Clostridia. Finally, the binning procedure discovered 15 putative genomes which dominated the microbial community of the two samples, and included representatives of genera Erwinia, Pantoea, Pseudomonas, Clostridium, Paenibacillus, and of orders Lactobacillales and Actinomycetales. These results provide insights into the microbial consortium of Corvina withered berries and reveal relevant variations attributable to post-harvest withering conditions, underling how WMS could open novel perspectives in the knowledge and management of the withering process of Corvina, with an impact on the winemaking of important Italian wines. en
dc.description.sponsorship European Social Fund (Reference 1695/101/9/1148/2013) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.rights © 2016 Salvetti, Campanaro, Campedelli, Fracchetti, Gobbi, Tornielli, Torriani and Felis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Withered grape en
dc.subject Post-harvest en
dc.subject Microbiome en
dc.subject Microbial en
dc.subject Diversity en
dc.subject Yeasts en
dc.subject Moulds en
dc.subject Bacteria en
dc.subject Metagenomics en
dc.title Whole-metagenome-sequencing-based community profiles of Vitis vinifera L. cv. Corvina berries withered in two post-harvest conditions en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Elisa Salvetti, APC Microbiome Institute, University College Cork, Cork, Ireland T: +353 21 490 3000 E: elisa.salvetti@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder European Social Fund en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.internal.copyrightchecked !!CORA!! en
dc.internal.IRISemailaddress elisa.salvetti@ucc.ie en


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© 2016 Salvetti, Campanaro, Campedelli, Fracchetti, Gobbi, Tornielli, Torriani and Felis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2016 Salvetti, Campanaro, Campedelli, Fracchetti, Gobbi, Tornielli, Torriani and Felis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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