Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

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dc.contributor.author Batista-García, Ramón Alberto
dc.contributor.author Sutton, Thomas
dc.contributor.author Jackson, Stephen A.
dc.contributor.author Tovar-Herrera, Omar Eduardo
dc.contributor.author Balcázar-López, Edgar
dc.contributor.author Sánchez-Carbente, María del Rayo
dc.contributor.author Sánchez-Reyes, Ayixon
dc.contributor.author Dobson, Alan D. W.
dc.contributor.author Folch-Mallol, Jorge Luis
dc.date.accessioned 2017-04-28T11:59:45Z
dc.date.available 2017-04-28T11:59:45Z
dc.date.issued 2017-03
dc.identifier.citation Batista-García, R. A., Sutton, T., Jackson, S. A., Tovar-Herrera, O. E., Balcázar-López, E., Sánchez-Carbente, M. d. R., Sánchez-Reyes, A., Dobson, A. D. W. and Folch-Mallol, J. L. (2017) 'Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani', PLOS ONE, 12(3), pp. e0173750. doi:10.1371/journal.pone.0173750 en
dc.identifier.volume 12 en
dc.identifier.issued 3 en
dc.identifier.startpage e0173750-1 en
dc.identifier.endpage e0173750-30 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/3903
dc.identifier.doi 10.1371/journal.pone.0173750
dc.description.abstract Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less information is available on fungal biodiversity associated with these sponges. Following screening of fourteen fungi isolated from the deep-sea sponge Stelletta normani sampled at a depth of 751 metres, three halotolerant strains (TS2, TS11 and TS12) were identified which displayed high CMCase and xylanase activities. Molecular based taxonomic approaches identified these strains as Cadophora sp. TS2, Emericellopsis sp. TS11 and Pseudogymnoascus sp. TS 12. These three fungi displayed psychrotolerance and halotolerant growth on CMC and xylan as sole carbon sources, with optimal growth rates at 20°C. They produced CMCase and xylanase activities, which displayed optimal temperature and pH values of between 50–70°C and pH 5–8 respectively, together with good thermostability and halotolerance. In solid-state fermentations TS2, TS11 and TS12 produced CMCases, xylanases and peroxidase/phenol oxidases when grown on corn stover and wheat straw. This is the first time that CMCase, xylanase and peroxidase/phenol oxidase activities have been reported in these three fungal genera isolated from a marine sponge. Given the biochemical characteristics of these ligninolytic enzymes it is likely that they may prove useful in future biomass conversion strategies involving lignocellulosic materials. en
dc.description.sponsorship Marine Institute (Beaufort Marine Research Award, part of the Sea Change Strategy and the Strategy for Science Technology and Innovation (2006±2012), with the support of The Marine Institute under the Marine Research Sub-Programme of the National Development Plan 2007±2013); National Council for Science and Technology (CONACyT)-Mexico (Project CB-153789; and PRODEP-SEP Project UAEMOR-PTC-333) Europe Molecular Biology Organization (EMBO: ASTF503-2013); Department of Agriculture, Food and the Marine (DAFF11/F/009 MabS); en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.relation.uri http://dx.doi.org/10.1371%2Fjournal.pone.0173750
dc.rights © 2017 Batista-García et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Fungi en
dc.subject Sponges en
dc.subject Cellulases en
dc.subject Phylogenetics en
dc.subject Straw en
dc.subject Wheat en
dc.subject Maize en
dc.subject Phylogenetic analysis en
dc.title Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Alan Dobson, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: a.dobson@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-04-28T11:42:36Z
dc.description.version Published Version en
dc.internal.rssid 392962371
dc.contributor.funder Seventh Framework Programme en
dc.contributor.funder Marine Institute en
dc.contributor.funder Consejo Nacional de Ciencia y Tecnología en
dc.contributor.funder European Molecular Biology Organization en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Plos One en
dc.internal.copyrightchecked No en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress a.dobson@ucc.ie en
dc.identifier.articleid e0173750
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA en


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© 2017 Batista-García et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2017 Batista-García et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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