Developmental age and UV-B exposure co-determine antioxidant capacity and flavonol accumulation in Arabidopsis leaves
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Accepted version
Date
2017-05-17
Authors
Csepregi, Kristóf
Coffey, Aoife M.
Cunningham, Natalie
Prinsen, Els
Hideg, Éva
Jansen, Marcel A. K.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Published Version
Abstract
Developmental age is an important determinant of plant stress responses. In this study the importance of “within-individual-heterogeneity” of developmental age for plant UV-B responses was quantified. Arabidopsis thaliana rosettes were raised under indoor conditions, and the responses of leaves at different developmental stages were compared following exposure to supplemental UV-B radiation. Exposure to a low dose of UV-B had positive effects on concentrations of UV-absorbing pigments, quercetins and kaempferols as well as total antioxidant activity measured. Unlike UV-B, developmental age had a substantial effect on photochemistry, and especially energy dissipation. Younger leaves display relatively strong regulated dissipation, while older leaves show more non-regulated, non-photochemical energy dissipation. Developmental age also impacted on concentrations of UV-absorbing compounds, and antioxidant activity. In fact, developmental variation matched, or even exceeded the UV-induced response for these two parameters. Thus, pooling of rosette leaves is not necessarily a good strategy to visualise plant UV-responses. Rather, to fully understand plant UV-responses in a developmental context it is important to advance reporter technologies for physiological studies, including spin-trap technology to visualise in planta ROS and ROS-defences, and fluorescence excitation screening technology and chromogenic assays for in planta visualisation of specific UV-absorbing pigments.
Description
Keywords
Antioxidant , Arabidopsis , Development , Flavonol , Photochemistry , Ultraviolet-B
Citation
Csepregi, K., Coffey, A., Cunningham, N., Prinsen, E., Hideg, É. and Jansen, M. A. K. (2017) 'Developmental age and UV-B exposure co-determine antioxidant capacity and flavonol accumulation in Arabidopsis leaves', Environmental and Experimental Botany, 140, pp. 19-25. doi:10.1016/j.envexpbot.2017.05.009