Cystathione gamma lyase/hydrogen sulphide pathway up regulation enhances the responsiveness of ?1A and ?1B-adrenoreceptors in the kidney of rats with left ventricular hypertrophy

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dc.contributor.author Ahmad, Ashfaq
dc.contributor.author Sattar, Munavvar A.
dc.contributor.author Azam, Maleeha
dc.contributor.author Abdulla, Mohammed H.
dc.contributor.author Khan, Safia A.
dc.contributor.author Hashmi, Fayyaz
dc.contributor.author Abdullah, Nor A.
dc.contributor.author Johns, Edward J.
dc.date.accessioned 2017-06-21T11:01:26Z
dc.date.available 2017-06-21T11:01:26Z
dc.date.issued 2016-05-18
dc.identifier.citation Ahmad, A., Sattar, M. A., Azam, M., Abdulla, M. H., Khan, S. A., Hashmi, F., Abdullah, N. A. and Johns, E. J. (2016) 'Cystathione gamma lyase/Hydrogen sulphide pathway up regulation enhances the responsiveness of α1A and α1B-adrenoreceptors in the kidney of rats with left ventricular hypertrophy', PLoS ONE, 11(5), e0154995 (23pp). doi: 10.1371/journal.pone.0154995 en
dc.identifier.volume 11
dc.identifier.issued 5
dc.identifier.startpage 1
dc.identifier.endpage 23
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/4135
dc.identifier.doi 10.1371/journal.pone.0154995
dc.description.abstract The purpose of the present study was to investigate the interaction between H2S and NO (nitric oxide) in the kidney and to evaluate its impact on the functional contribution of ?1A and ?1B-adrenoreceptors subtypes mediating the renal vasoconstriction in the kidney of rats with left ventricular hypertrophy (LVH). In rats the LVH induction was by isoprenaline administration and caffeine in the drinking water together with intraperitoneal administration of H2S. The responsiveness of ?1A and ?1B to exogenous noradrenaline, phenylephrine and methoxaminein the absence and presence of 5-methylurapidil (5-MeU) and chloroethylclonidine (CEC) was studied. Cystathione gamma lyase (CSE), cystathione ? synthase (CBS), 3-mercaptopyruvate sulphar transferase (3-MST) and endothelial nitric oxide synthase (eNOS) were quantified. There was significant up regulation of CSE and eNOS in the LVH-H2S compared to the LVH group (P<0.05). Baseline renal cortical blood perfusion (RCBP) was increased (P<0.05) in the LVH-H2S compared to the LVH group. The responsiveness of ?1A-adrenergic receptors to adrenergic agonists was increased (P<0.05) after administration of low dose 5-Methylurapidil in the LVH-H2S group while ?1B-adrenergic receptors responsiveness to adrenergic agonists were increased (P<0.05) by both low and high dose chloroethylclonidine in the LVH-H2S group. Treatment of LVH with H2S resulted in up-regulation of CSE/H2S, CBS, and 3-MST and eNOS/NO/cGMP pathways in the kidney. These up regulation of CSE/H2S, CBS, and 3-MST and eNOS/NO/cGMP pathways enhanced the responsiveness of ?1A and ?1B-adrenoreceptors subtypes to adrenergic agonists in LVH-H2S. These findings indicate an important role for H2S in modulating deranged signalling in the renal vasculature resulting from LVH development. en
dc.description.sponsorship Institute of Postgraduate Studies (APEX (1002/JHEA/ATSG4001); Universiti Sains Malaysia (grant no.1001/PFARMASI/815078 and HIR grant UM.0000069/HIR.C3) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLoS en
dc.relation.uri http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154995
dc.rights © 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Kidneys en
dc.subject Blood en
dc.subject Vasoconstriction en
dc.subject RNA extraction en
dc.subject Renal system en
dc.subject Urine en
dc.subject Vasodilation en
dc.subject Renal physiology en
dc.title Cystathione gamma lyase/hydrogen sulphide pathway up regulation enhances the responsiveness of ?1A and ?1B-adrenoreceptors in the kidney of rats with left ventricular hypertrophy en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Mohammed H. Abdulla, Physiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: m.abdulla@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Institute of Postgraduate Studies, Universiti Sains Malaysia
dc.contributor.funder Universiti Sains Malaysia
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.IRISemailaddress m.abdulla@ucc.ie en
dc.identifier.articleid e0154995


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© 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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