Up regulation of cystathione ? lyase and Hydrogen sulphide in the myocardium inhibits the progression of isoproterenol-caffeine induced left ventricular hypertrophy in wistar kyoto rats

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dc.contributor.author Ahmad, Ashfaq
dc.contributor.author Sattar, Munavvar A.
dc.contributor.author Rathore, Hassaan A.
dc.contributor.author Abdulla, Mohammed H.
dc.contributor.author Khan, Safia A.
dc.contributor.author Azam, Maleeha
dc.contributor.author Abdullah, Nor A.
dc.contributor.author Johns, Edward J.
dc.date.accessioned 2017-06-21T11:01:26Z
dc.date.available 2017-06-21T11:01:26Z
dc.date.issued 2016-03-10
dc.identifier.citation Ahmad, A., Sattar, M. A., Rathore, H. A., Abdulla, M. H., Khan, S. A., Azam, M., Abdullah, N. A. and Johns, E. J. (2016) 'Up Regulation of cystathione ? lyase and hydrogen sulphide in the myocardium inhibits the progression of isoproterenol–caffeine induced left ventricular hypertrophy in Wistar Kyoto Rats', PLoS ONE, 11(3), e0150137 (20pp). doi: 10.1371/journal.pone.0150137 en
dc.identifier.volume 11
dc.identifier.issued 3
dc.identifier.startpage 1
dc.identifier.endpage 20
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/4137
dc.identifier.doi 10.1371/journal.pone.0150137
dc.description.abstract Hydrogen sulphide (H2S) is an emerging molecule in many cardiovascular complications but its role in left ventricular hypertrophy (LVH) is unknown. The present study explored the effect of exogenous H2S administration in the regression of LVH by modulating oxidative stress, arterial stiffness and expression of cystathione ? lyase (CSE) in the myocardium. Animals were divided into four groups: Control, LVH, Control-H2S and LVH-H2S. LVH was induced by administering isoprenaline (5mg/kg, every 72 hours, S/C) and caffeine in drinking water (62mg/L) for 2 weeks. Intraperitoneal NaHS, 56?M/kg/day for 5 weeks, was given as an H2S donor. Myocardial expression of Cystathione ? lyase (CSE) mRNA was quantified using real time polymerase chain reaction (qPCR).There was a 3 fold reduction in the expression of myocardial CSE mRNA in LVH but it was up regulated by 7 and 4 fold in the Control-H2S and LVH-H2S myocardium, respectively. Systolic blood pressure, mean arterial pressure, pulse wave velocity were reduced (all P<0.05) in LVH-H2S when compared to the LVH group. Heart, LV weight, myocardial thickness were reduced while LV internal diameter was increased (all P<0.05) in the LVH-H2S when compared to the LVH group. Exogenous administration of H2S in LVH increased superoxide dismutase, glutathione and total antioxidant capacity but significantly reduced (all P<0.05) plasma malanodialdehyde in the LVH-H2S compared to the LVH group. The renal cortical blood perfusion increased by 40% in LVH-H2S as compared to the LVH group. Exogenous administration of H2S suppressed the progression of LVH which was associated with an up regulation of myocardial CSE mRNA/ H2S and a reduction in pulse wave velocity with a blunting of systemic hemodynamic. This CSE/H2S pathway exhibits an antihypertrophic role by antagonizing the hypertrophic actions of angiotensin II(Ang II) and noradrenaline (NA) but attenuates oxidative stress and improves pulse wave velocity which helps to suppress LVH. Exogenous administration of H2S augmented the reduced renal cortical blood perfusion in the LVH state. en
dc.description.sponsorship Institute of Postgraduate Studies (APEX (1002/JHEA/ATSG4001); Universiti Sains Malaysia (grant no. 1001/PFARMASI/815078, HIR grant UM.0000069/HIR.C3) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLoS en
dc.relation.uri http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150137
dc.rights © 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Blood plasma en
dc.subject Antioxidants en
dc.subject Myocardium en
dc.subject Oxidative stress en
dc.subject Heart en
dc.subject Blood pressure en
dc.subject Cardiac hypertrophy en
dc.subject Superoxide dismutase en
dc.title Up regulation of cystathione ? lyase and Hydrogen sulphide in the myocardium inhibits the progression of isoproterenol-caffeine induced left ventricular hypertrophy in wistar kyoto rats en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Mohammed H. Abdulla, Physiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: m.abdulla@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Institute of Postgraduate Studies, Universiti Sains Malaysia
dc.contributor.funder Universiti Sains Malaysia
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.IRISemailaddress m.abdulla@ucc.ie en
dc.identifier.articleid e0150137


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© 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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