Molecular signature of Pseudomonas aeruginosa with simultaneous nanomolar detection of quorum sensing signaling molecules at a Boron-Doped diamond electrode

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dc.contributor.author Buzid, Alyah
dc.contributor.author Shang, Fengjun
dc.contributor.author Reen, F. Jerry
dc.contributor.author Ó Muimhneacháin, Eoin
dc.contributor.author Clarke, Sarah L.
dc.contributor.author Zhou, Lin
dc.contributor.author Luong, John H. T.
dc.contributor.author O'Gara, Fergal
dc.contributor.author McGlacken, Gerard P.
dc.contributor.author Glennon, Jeremy D.
dc.date.accessioned 2017-06-22T13:55:53Z
dc.date.available 2017-06-22T13:55:53Z
dc.date.issued 2016-07-18
dc.identifier.citation Buzid, A., Shang, F., Reen, F. J., Muimhneacháin, E. Ó., Clarke, S. L., Zhou, L., Luong, J. H. T., O’Gara, F., McGlacken, G. P. and Glennon, J. D. (2016) 'Molecular Signature of Pseudomonas aeruginosa with Simultaneous Nanomolar Detection of Quorum Sensing Signaling Molecules at a Boron-Doped Diamond Electrode', Scientific Reports, 6, 30001 (9pp). doi: 10.1038/srep30001 en
dc.identifier.volume 6
dc.identifier.startpage 1
dc.identifier.endpage 9
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10468/4168
dc.identifier.doi 10.1038/srep30001
dc.description.abstract Electroanalysis was performed using a boron-doped diamond (BDD) electrode for the simultaneous detection of 2-heptyl-3-hydroxy-4-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ) and pyocyanin (PYO). PQS and its precursor HHQ are two important signal molecules produced by Pseudomonas aeruginosa, while PYO is a redox active toxin involved in virulence and pathogenesis. This Gram-negative and opportunistic human pathogen is associated with a hospital-acquired infection particularly in patients with compromised immunity and is the primary cause of morbidity and mortality in cystic fibrosis (CF) patients. Early detection is crucial in the clinical management of this pathogen, with established infections entering a biofilm lifestyle that is refractory to conventional antibiotic therapies. Herein, a detection procedure was optimized and proven for the simultaneous detection of PYO, HHQ and PQS in standard mixtures, biological samples, and P. aeruginosa spiked CF sputum samples with remarkable sensitivity, down to nanomolar levels. Differential pulse voltammetry (DPV) scans were also applicable for monitoring the production of PYO, HHQ and PQS in P. aeruginosa PA14 over 8 h of cultivation. The simultaneous detection of these three compounds represents a molecular signature specific to this pathogen. en
dc.description.sponsorship Science Foundation Ireland/Enterprise Ireland (Technology Innovation Development Award (TIDA) (SFI/12/TIDA/B2405). Science Foundation Ireland (SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438; SFI/12/IP/1315; SFI/09/RFP/CHS2353; SSPC2 12/RC/2275; 08/SRC/B1412); Department of Agriculture and Food (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council (Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647); GOIPG/2013/336); Health Research Board/Irish Thoracic Society (MRCG-2014-6); Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013); University College Cork (Strategic Research Fund Ph.D. Studentship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Nature Publishing Group en
dc.relation.uri https://www.nature.com/articles/srep30001
dc.rights © 2016, Buzid et al. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Diagnostic markers en
dc.subject Medical and clinical diagnostics en
dc.title Molecular signature of Pseudomonas aeruginosa with simultaneous nanomolar detection of quorum sensing signaling molecules at a Boron-Doped diamond electrode en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Jeremy D. Glennon, Chemistry and Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, Ireland. +353-21-490-3000 E-mail: j.glennon@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Enterprise Ireland
dc.contributor.funder Department of Agriculture, Food and the Marine
dc.contributor.funder Irish Research Council
dc.contributor.funder European Commission
dc.contributor.funder Marine Institute
dc.contributor.funder Teagasc
dc.contributor.funder Health Research Board
dc.contributor.funder Irish Thoracic Society
dc.contributor.funder University College Cork
dc.description.status Peer reviewed en
dc.identifier.journaltitle Scientific Reports en
dc.internal.IRISemailaddress j.glennon@ucc.ie en
dc.identifier.articleid 30001
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATION
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE


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© 2016, Buzid et al. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as © 2016, Buzid et al. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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