Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification

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dc.contributor.author Stefanovic, Ewelina
dc.contributor.author Kilcawley, Kieran N.
dc.contributor.author Rea, Mary C.
dc.contributor.author Fitzgerald, Gerald F.
dc.contributor.author McAuliffe, Olivia
dc.date.accessioned 2017-08-18T10:58:44Z
dc.date.available 2017-08-18T10:58:44Z
dc.date.issued 2017-04-04
dc.identifier.citation Stefanovic, E., Kilcawley, K. N., Rea, M. C., Fitzgerald, G. F. and McAuliffe, O. (2017) 'Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification', Journal of Applied Microbiology, 122(5), pp. 1245–1261. doi:10.1111/jam.13420 en
dc.identifier.volume 122 en
dc.identifier.issued 5 en
dc.identifier.startpage 1245 en
dc.identifier.endpage 1261 en
dc.identifier.issn 1365-2672
dc.identifier.uri http://hdl.handle.net/10468/4469
dc.identifier.doi 10.1111/jam.13420
dc.description.abstract Aims: The Lactobacillus casei group represents a widely explored group of lactic acid bacteria, characterized by a high level of biodiversity. In this study, the genetic and phenotypic diversity of a collection of more than 300 isolates of the Lact. casei group and their potential to produce volatile metabolites important for flavour development in dairy products, was examined. Methods and Results: Following confirmation of species by 16S rRNA PCR, the diversity of the isolates was determined by pulsed-field gel electrophoresis. The activities of enzymes involved in the proteolytic cascade were assessed and significant differences among the strains were observed. Ten strains were chosen based on the results of their enzymes activities and they were analysed for their ability to produce volatiles in media with increased concentrations of a representative aromatic, branched chain and sulphur amino acid. Volatiles were assessed using gas chromatography coupled with mass spectrometry. Strain-dependent differences in the range and type of volatiles produced were evident. Conclusions: Strains of the Lact. casei group are characterized by genetic and metabolic diversity which supports variability in volatile production. Significance and Impact of the Study: This study provides a screening approach for the knowledge-based selection of strains potentially enabling flavour diversification in fermented dairy products. en
dc.description.sponsorship Teagasc (Walsh Fellowship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Inc. en
dc.rights © 2017, the Society for Applied Microbiology. This is the peer reviewed version of the following article: Stefanovic, E., Kilcawley, K. N., Rea, M. C., Fitzgerald, G. F. and McAuliffe, O. (2017) 'Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification', Journal of Applied Microbiology, 122(5), pp. 1245–1261, which has been published in final form at http://dx.doi.org/10.1111/jam.13420. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. en
dc.subject Dairy en
dc.subject Diversity en
dc.subject Lactobacillus en
dc.subject PFGE en
dc.subject Proteinase en
dc.title Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Gerald F. Fitzgerald, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: g.fitzgerald@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2018-04-04
dc.date.updated 2017-08-18T10:48:01Z
dc.description.version Accepted Version en
dc.internal.rssid 407466559
dc.contributor.funder Teagasc en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Applied Microbiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress g.fitzgerald@ucc.ie en


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