Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells

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dc.contributor.author Rahme, Kamil
dc.contributor.author Guo, Jianfeng
dc.contributor.author Biswas, Subhajit
dc.contributor.author O'Driscoll, Caitríona M.
dc.contributor.author Holmes, Justin D.
dc.date.accessioned 2017-09-20T15:05:50Z
dc.date.available 2017-09-20T15:05:50Z
dc.date.issued 2017-05
dc.identifier.citation Rahme, K., Guo, J., Biswas, S., O'Driscoll, C. and Holmes, J. (2017) Branched PEI Capped Gold Nanoparticles in Water for siRNA Delivery to Cancer Cells, TechConnect 2017 Washington, DC, USA, 14 -17 May, in Advanced Materials: TechConnect Briefs, Chapter 4, pp. 159-162. isbn: 978-0-9975117-8-9 en
dc.identifier.startpage 159 en
dc.identifier.endpage 162 en
dc.identifier.isbn 978-0-9975117-8-9
dc.identifier.uri http://hdl.handle.net/10468/4752
dc.description.abstract Herein we describe a simple method for the synthesis of different sizes of polyethylenimine-capped gold nanoparticles (AuNPs-PEI) in water and assess their potential to deliver siRNA or other therapeutic agents to cancer cells. AuNP-PEI with diameters ranging between 25-150 nm have been synthesised in aqueous solutions using PEI (25 KD and 2KD) as capping ligands and using hydroxylamine-O-sulfonic acid or ascorbic acid as reducing agents. Different parameters were found to affect the final size of nanoparticles core (i.e. gold salt concentrations, PEI molecular weight/concentrations, and temperature). The obtained AuNP-PEIs were fully characterized using UV-visible spectroscopy, Electron Microscopy (EM), and Dynamic Light Scattering (DLS). UV-visible spectra clearly showed that the synthesized particles have size dependent optical properties with a plasmon band shift to longer wavelengths, as the size of the AuNP core was increased. In addition, DLS analysis indicated that all samples were nearly monodisperse with one size distribution and a polydispersity index (PDI) of about 0.15 (Std 0.03). EM analysis indicated that AuNPs-PEI were nearly spherical in shape. Zeta (ζ) potential measurements showed that all AuNPs-PEI samples were positively charged with a ζ-potential in the range of 38 (Std 5 mV), leading to a very high stability of the colloidal solution for several months when stocked at 4 C. Furthermore, the potential application of AuNP-PEIs in siRNA delivery to PC-3 prostate cancer cells was investigated. The ability of AuNP-PEIs to complex siRNA was analysed by gel electrophoresis. Results indicated that AuNP-PEI 2KD and AuNP-PEI 25KD could complex siRNA at MR0.5 and MR0.25 onwards, respectively, suggesting that AuNP-PEI 25KD has a better siRNA binding capacity than AuNP-PEI 2KD. Cellular uptakes were also performed using PC-3 cancer cells. Results following 24 h incubation indicated that AuNP-PEI 25KD achieved significantly higher fluorescein-positive cells (~ 98%) relative to that of AuNP-PEI 2KD (~ 5%), suggesting that AuNP-PEI 25KD, but not AuNP-PEI 2KD, could deliver siRNA into cells. Finally, we have also demonstrated that the surface of AuNPs-PEI can be further conjugated with thiolated polyethylene glycol (SH-PEG) onto AuNPs surface. Moreover, we have previously showed that covalently bonded targeting ligand (such as Anisic Acid or Folic acid) could be also chemically grafted on PEI, leading therefore to a multifunctional nanoparticle that may be promising in the field of Nanobiotechnology and Nanomedicine. en
dc.description.sponsorship Conseil National de la Recherche Scientifique, National Council for Scientific Research Lebanon (CNRS-L-GRP2015-3538); Science Foundation Ireland ((AMBER SFI funded Advanced Materials and BioEngineering Research Centre), (SFI Grant 12/RC/2278)) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher TechConnect en
dc.relation.ispartof TechConnect 2017
dc.relation.uri https://www.techconnect.org/proceedings/paper.html?volume=TCB2017v1&chapter=4&paper=349
dc.rights © 2017 TechConnect. All rights reserved. en
dc.subject Gold nanoparticles en
dc.subject Polymers en
dc.subject siRNA delivery en
dc.subject Cancer en
dc.title Branched PEI capped gold nanoparticles in water for siRNA delivery to cancer cells en
dc.type Conference item en
dc.internal.authorcontactother Justin D. Holmes, Chemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.holmes@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2017-07-13T15:14:11Z
dc.description.version Published Version en
dc.internal.rssid 402759574
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Conseil National de la Recherche Scientifique
dc.description.status Peer reviewed en
dc.identifier.journaltitle Advanced Materials: TechConnect Briefs en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.conferencelocation Washington, DC, USA en
dc.internal.IRISemailaddress j.holmes@ucc.ie en


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