Avoidance of reporter assay distortions from fused dual reporters

Show simple item record

dc.contributor.author Loughran, Gary
dc.contributor.author Howard, Michael T.
dc.contributor.author Firth, Andrew E.
dc.contributor.author Atkins, John F.
dc.date.accessioned 2017-09-26T11:39:19Z
dc.date.available 2017-09-26T11:39:19Z
dc.date.issued 2017
dc.identifier.citation Loughran, G., Howard, M. T., Firth, A. E. and Atkins, J. F. (2017) 'Avoidance of reporter assay distortions from fused dual reporters', RNA, 23(8) (6pp). doi: 10.1261/rna.061051.117 en
dc.identifier.volume 23
dc.identifier.issued 8
dc.identifier.startpage 1285
dc.identifier.endpage 1289
dc.identifier.issn 1355-8382
dc.identifier.issn 1469-9001
dc.identifier.uri http://hdl.handle.net/10468/4784
dc.identifier.doi 10.1261/rna.061051.117
dc.description.abstract Positioning test sequences between fused reporters permits monitoring of both translation levels and framing, before and after the test sequence. Many studies, including those on recoding such as productive ribosomal frameshifting and stop codon readthrough, use distinguishable luciferases or fluorescent proteins as reporters. Occasional distortions, due to test sequence product interference with the individual reporter activities or stabilities, are here shown to be avoidable by the introduction of tandem StopGo sequences (2A) flanking the test sequence. Using this new vector system (pSGDluc), we provide evidence for the use of a 3' stem-loop stimulator for ACP2 readthrough, but failed to detect the reported VEGFA readthrough. en
dc.description.sponsorship National Institutes of Health (R01GM114291; R21ES022716); Wellcome Trust (106207) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Cold Spring Harbor Lab Press en
dc.relation.uri http://rnajournal.cshlp.org/content/23/8/1285
dc.rights © 2017, Loughran et al. Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Recoding en
dc.subject Frameshifting en
dc.subject Readthrough en
dc.subject Dual luciferase en
dc.subject StopGo en
dc.subject VEGF-Ax en
dc.subject Cleavage en
dc.subject Stop en
dc.subject 2A en
dc.subject Codon en
dc.subject Translation en
dc.subject Polyprotein en
dc.subject Aphthovirus en
dc.subject Readthrough en
dc.subject Fusion en
dc.title Avoidance of reporter assay distortions from fused dual reporters en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Gary Loughran, Biochemistry and Cell Biology, University College Cork, Cork, Ireland. +353-21-490-3000. Email: g.loughran@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000405999100013
dc.contributor.funder European Research Council
dc.contributor.funder Wellcome Trust
dc.contributor.funder National Institutes of Health
dc.contributor.funder Science Foundation Ireland
dc.description.status Peer reviewed en
dc.identifier.journaltitle RNA en
dc.internal.IRISemailaddress g.loughran@ucc.ie en
dc.internal.IRISemailaddress j.atkins@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IP/1492/IE/Using ribosome profiling to study translation initiation/elongation and facilitate optimization of protein synthesis/
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1853/IE/Dynamic redefinition of codons: From antivirals to an essential micronutrient/
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::ERC::ERC-COG/646891/EU/Systematic discovery of functional elements in RNA virus genomes: an Encyclopedia of RNA Virus Elements/ERVE


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2017, Loughran et al. Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. Except where otherwise noted, this item's license is described as © 2017, Loughran et al. Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement