Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates

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dc.contributor.author Nogacka, Alicja
dc.contributor.author Salazar, Nuria
dc.contributor.author Suarez, Marta
dc.contributor.author Milani, Christian
dc.contributor.author Arboleya, Silvia
dc.contributor.author Solis, Gonzalo
dc.contributor.author Fernandez, Nuria
dc.contributor.author Alaez, Lidia
dc.contributor.author Hernandez-Barranco, Ana M.
dc.contributor.author de los Reyes-Gavilan, Clara G.
dc.contributor.author Ventura, Marco
dc.contributor.author Gueimonde, Miguel
dc.date.accessioned 2017-09-26T11:39:22Z
dc.date.available 2017-09-26T11:39:22Z
dc.date.issued 2017
dc.identifier.citation Nogacka, A., Salazar, N., Suárez, M., Milani, C., Arboleya, S., Solís, G., Fernández, N., Alaez, L., Hernández-Barranco, A. M., de los Reyes-Gavilán, C. G., Ventura, M. and Gueimonde, M. (2017) 'Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates', Microbiome, 5(1), 93 (10pp). doi: 10.1186/s40168-017-0313-3 en
dc.identifier.volume 5
dc.identifier.issn 2049-2618
dc.identifier.uri http://hdl.handle.net/10468/4802
dc.identifier.doi 10.1186/s40168-017-0313-3
dc.description.abstract Background: Disturbances in the early establishment of the intestinal microbiota may produce important implications for the infant's health and for the risk of disease later on. Different perinatal conditions may be affecting the development of the gut microbiota. Some of them, such as delivery mode or feeding habits, have been extensively assessed whereas others remain to be studied, being critical to identify their impact on the microbiota and, if any, to minimize it. Antibiotics are among the drugs most frequently used in early life, the use of intrapartum antimicrobial prophylaxis (IAP), present in over 30% of deliveries, being the most frequent source of exposure. However, our knowledge on the effects of IAP on the microbiota establishment is still limited. The aim of the present work was to evaluate the impact of IAP investigating a cohort of 40 full-term vaginally delivered infants born after an uncomplicated pregnancy, 18 of which were born from mothers receiving IAP. Results: Fecal samples were collected at 2, 10, 30, and 90 days of age. We analyzed the composition of the fecal microbiota during the first 3 months of life by 16S rRNA gene sequencing and quantified fecal short chain fatty acids by gas chromatography. The presence of genes for resistance to antibiotics was determined by PCR in the samples from 1-month-old infants. Our results showed an altered pattern of intestinal microbiota establishment in IAP infants during the first weeks of life, with lower relative proportions of Actinobacteria and Bacteroidetes and increased of Preoteobacteria and Firmicutes. A delay in the increase on the levels of acetate was observed in IAP infants. The analyses of specific antibiotic resistance genes showed a higher occurrence of some beta-lactamase coding genes in infants whose mothers received IAP. Conclusions: Our results indicate an effect of IAP on the establishing early microbiota during the first months of life, which represent a key moment for the development of the microbiota-induced host homeostasis. Understanding the impact of IAP in the gut microbiota development is essential for developing treatments to minimize it, favoring a proper gut microbiota development in IAP-exposed neonates. en
dc.description.sponsorship Ministerio de Economía y Competitividad (PCIN-2015-233; AGL2013-43770R); Consejo Superior de Investigaciones Científicas (Unit of Information Resources for Research); Plan Regional de Investigación del Principado de Asturias (GRUPIN14-043) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Biomed Central Ltd en
dc.relation.uri https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0313-3
dc.rights © 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Intestinal microbiota en
dc.subject Microbiome en
dc.subject Antibiotics en
dc.subject Neonate en
dc.subject Intrapartum antimicrobial prophylaxis en
dc.subject Antibiotics en
dc.subject Infant gut microbiota en
dc.subject Group B streptococcus en
dc.subject Early life en
dc.subject Fecal microbiota en
dc.subject Children en
dc.subject Health en
dc.subject Establishment en
dc.subject Consequences en
dc.subject Colonization en
dc.subject Physiology en
dc.title Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Silvia Arboleya, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. +353-21-490-3000 Email: en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000407724100002
dc.contributor.funder Gobierno del Principado de Asturias
dc.contributor.funder Consejo Superior de Investigaciones Científicas
dc.contributor.funder Ministero dell’Istruzione, dell’Università e della Ricerca
dc.contributor.funder Ministerio de Economía y Competitividad
dc.description.status Peer reviewed en
dc.identifier.journaltitle Microbiome en
dc.internal.IRISemailaddress silvia.arboleya@teagasc.ie en
dc.identifier.articleid 93


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© 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Except where otherwise noted, this item's license is described as © 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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