Two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheL

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dc.contributor.author Gurvich, Olga L.
dc.contributor.author Näsvall, S. Joakim
dc.contributor.author Baranov, Pavel V.
dc.contributor.author Björk, Glenn R.
dc.contributor.author Atkins, John F.
dc.date.accessioned 2017-11-14T13:24:31Z
dc.date.available 2017-11-14T13:24:31Z
dc.date.issued 2011
dc.identifier.citation Gurvich, O. L., Näsvall, S. J., Baranov, P. V., Björk, G. R. and Atkins, J. F. (2011) 'Two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheL', Nucleic Acids Research, 39(8), pp. 3079-3092. doi: 10.1093/nar/gkq1272 en
dc.identifier.volume 39
dc.identifier.issued 8
dc.identifier.startpage 3079
dc.identifier.endpage 3092
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10468/5025
dc.identifier.doi 10.1093/nar/gkq1272
dc.description.abstract The bacterial pheL gene encodes the leader peptide for the phenylalanine biosynthetic operon. Translation of pheL mRNA controls transcription attenuation and, consequently, expression of the downstream pheA gene. Fifty-three unique pheL genes have been identified in sequenced genomes of the gamma subdivision. There are two groups of pheL genes, both of which are short and contain a run(s) of phenylalanine codons at an inteRNAl position. One group is somewhat diverse and features different termination and 5'-flanking codons. The other group, mostly restricted to Enterobacteria and including Escherichia coli pheL, has a conserved nucleotide sequence that ends with UUC_CCC_UGA. When these three codons in E. coli pheL mRNA are in the ribosomal E-, P- and A-sites, there is an unusually high level, 15%, of +1 ribosomal frameshifting due to features of the nascent peptide sequence that include the penultimate phenylalanine. This level increases to 60% with a natural, heterologous, nascent peptide stimulator. Nevertheless, studies with different tRNA(Pro) mutants in Salmonella enterica suggest that frameshifting at the end of pheL does not influence expression of the downstream pheA. This finding of incidental, rather than utilized, frameshifting is cautionary for other studies of programmed frameshifting. en
dc.description.sponsorship National Institute of Health (GM079523) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Oxford University Press en
dc.relation.uri https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkq1272
dc.rights © 2010, the Authors. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://creativecommons.org/licenses/by-nc/2.5
dc.subject Translation termination en
dc.subject Transfer RNA en
dc.subject Salmonella-typhimurium en
dc.subject Nascent peptide en
dc.subject Messenger RNA en
dc.subject Attenuation regulation en
dc.subject Coding gap en
dc.subject In-vivo en
dc.subject Ribosome en
dc.subject Expression en
dc.title Two groups of phenylalanine biosynthetic operon leader peptides genes: a high level of apparently incidental frameshifting in decoding Escherichia coli pheL en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John F. Atkins, Biochemistry, University College Cork , Cork, Ireland T: +353-21-490-3000. E: j.atkins@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 348788041
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder National Institutes of Health
dc.contributor.funder Carl Tryggers Stiftelse för Vetenskaplig Forskning
dc.contributor.funder Svenska Forskningsrådet Formas
dc.description.status Peer reviewed en
dc.identifier.journaltitle Nucleic Acids Research en
dc.internal.IRISemailaddress j.atkins@ucc.ie en


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© 2010, the Authors. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © 2010, the Authors. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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