A beginner's guide to gene editing

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dc.contributor.author Harrison, Patrick T.
dc.contributor.author Hart, Stephen
dc.date.accessioned 2018-01-09T16:08:15Z
dc.date.available 2018-01-09T16:08:15Z
dc.date.issued 2017-12-28
dc.identifier.citation Harrison, P. T. and Hart, S. 'A beginner's guide to gene editing', Experimental Physiology, 103(4), pp. 439-448. doi:10.1113/EP086047 en
dc.identifier.volume 103
dc.identifier.issued 4
dc.identifier.startpage 439
dc.identifier.endpage 448
dc.identifier.issn 1469-445X
dc.identifier.uri http://hdl.handle.net/10468/5255
dc.identifier.doi 10.1113/EP086047
dc.description.abstract Genome editing enables precise changes to be made in the genome of living cells. The technique was originally developed in the 1980′s but largely limited to use in mice. The discovery that a targeted double stranded break (DSB) at a unique site in the genome, close to the site to be changed, could substantially increase the efficiency of editing raised the possibility of using the technique in a broader range of animal models and potentially human cells. But the challenge was to identify reagents that could create targeted breaks at a unique genomic location with minimal off-target effects. In 2005, the demonstration that programmable zinc finger nucleases (ZFNs) could perform this task, led to a number of proof-of-concept studies, but a limitation was the ease with which effective ZFNs could be produced. In 2009, the development of TAL-effector nucleases (TALENs) increased the specificity of gene editing and the ease of design and production. However, it wasn't until 2013 and the development of the CRISPR Cas9/guideRNA that gene editing became a research tool that any lab could use. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Wiley en
dc.rights © 2017 The Authors. Experimental Physiology © 2017 The Physiological Society. Published by Wiley. This is the peer reviewed version of the following article: Harrison, P. T. and Hart, S. 'A beginner's guide to gene editing', Experimental Physiology, 103(4), pp. 439-448, doi:10.1113/EP086047, which has been published in final form at http://dx.doi.org/10.1113/EP086047. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. en
dc.subject Cas9 en
dc.subject CRISPR en
dc.subject Cystic fibrosis en
dc.subject gRNA en
dc.subject TALEN en
dc.subject ZFN en
dc.subject Genome editing en
dc.subject Gene editing en
dc.title A beginner's guide to gene editing en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Patrick Harrison, Physiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: p.harrison@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2018-12-28
dc.date.updated 2018-01-09T15:57:59Z
dc.description.version Accepted Version en
dc.internal.rssid 421115574
dc.description.status Peer reviewed en
dc.identifier.journaltitle Experimental Physiology en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress p.harrison@ucc.ie en

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