Deficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in mice

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dc.contributor.author Robertson, Ruairi C.
dc.contributor.author Seira Oriach, Clara
dc.contributor.author Murphy, Kiera
dc.contributor.author Moloney, Gerard M.
dc.contributor.author Cryan, John F.
dc.contributor.author Dinan, Timothy G.
dc.contributor.author Ross, R. Paul
dc.contributor.author Stanton, Catherine
dc.date.accessioned 2018-01-10T16:35:03Z
dc.date.available 2018-01-10T16:35:03Z
dc.date.issued 2017-11-27
dc.identifier.citation Robertson, R. C., Seira Oriach, C., Murphy, K., Moloney, G. M., Cryan, J. F., Dinan, T. G., Ross, R. P. and Stanton, C. (2017) 'Deficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in mice', British Journal of Nutrition, 118(11), pp. 959-970. doi: 10.1017/S0007114517002999 en
dc.identifier.volume 118 en
dc.identifier.issued 11 en
dc.identifier.startpage 959 en
dc.identifier.endpage 970 en
dc.identifier.issn 0007-1145
dc.identifier.uri http://hdl.handle.net/10468/5267
dc.identifier.doi 10.1017/S0007114517002999
dc.description.abstract n-3 PUFA are lipids that play crucial roles in immune-regulation, cardio-protection and neurodevelopment. However, little is known about the role that these essential dietary fats play in modulating caecal microbiota composition and the subsequent production of functional metabolites. To investigate this, female C57BL/6 mice were assigned to one of three diets (control (CON), n-3 supplemented (n3+) or n-3 deficient (n3−)) during gestation, following which their male offspring were continued on the same diets for 12 weeks. Caecal content of mothers and offspring were collected for 16S sequencing and metabolic phenotyping. n3− male offspring displayed significantly less % fat mass than n3+ and CON. n-3 Status also induced a number of changes to gut microbiota composition such that n3− offspring had greater abundance of Tenericutes, Anaeroplasma and Coriobacteriaceae. Metabolomics analysis revealed an increase in caecal metabolites involved in energy metabolism in n3+ including α-ketoglutaric acid, malic acid and fumaric acid. n3− animals displayed significantly reduced acetate, butyrate and total caecal SCFA production. These results demonstrate that dietary n-3 PUFA regulate gut microbiota homoeostasis whereby n-3 deficiency may induce a state of disturbance. Further studies are warranted to examine whether these microbial and metabolic disturbances are causally related to changes in metabolic health outcomes. en
dc.description.sponsorship Health Research Board, Ireland (grant nos HRA_POR/2011/23 and HRA_POR/2012/32); en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Cambridge University Press (CUP) en
dc.relation.uri https://www.cambridge.org/core/article/deficiency-of-essential-dietary-n3-pufa-disrupts-the-caecal-microbiome-and-metabolome-in-mice/A8BCA9EB0AC0E9F67737796EF5FAA871
dc.rights © The Authors 2017 en
dc.subject Microbiome en
dc.subject Microbiota en
dc.subject n-3 PUFA en
dc.subject SCFA en
dc.subject Metabolomics en
dc.title Deficiency of essential dietary n-3 PUFA disrupts the caecal microbiome and metabolome in mice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John F Cryan, Department Of Anatomy & Neuroscience, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.cryan@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by the request of the publisher en
dc.check.date 2018-11-27
dc.date.updated 2018-01-10T15:47:19Z
dc.description.version Published Version en
dc.internal.rssid 421242689
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Health Research Board en
dc.contributor.funder Marine Institute en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Enterprise Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle British Journal of Nutrition en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress J.Cryan@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/ en


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