Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2

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dc.contributor.author Aprea, Giuseppe
dc.contributor.author Mullan, William Michael
dc.contributor.author Murru, Nicoletta
dc.contributor.author Fitzgerald, Gerald F.
dc.contributor.author Buonanno, Marialuisa
dc.contributor.author Cortesi, Maria Luisa
dc.contributor.author Prencipe, Vincenza Annunziata
dc.contributor.author Migliorat, Giacomo
dc.date.accessioned 2018-02-06T13:36:28Z
dc.date.available 2018-02-06T13:36:28Z
dc.date.issued 2017
dc.identifier.citation Aprea, G., Mullan, W. M., Murru, N., Fitzgerald, G., Buonanno, M., Cortesi, M. L., Prencipe, V. A. and Migliorati, G. (2017) 'Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2', Veterinaria Italiana, 53(3), pp. 207-214. doi: 10.12834/VetIt.315.1238.3 en
dc.identifier.volume 53
dc.identifier.issued 3
dc.identifier.startpage 207
dc.identifier.endpage 214
dc.identifier.issn 0505-401X
dc.identifier.uri http://hdl.handle.net/10468/5393
dc.identifier.doi 10.12834/VetIt.315.1238.3
dc.description.abstract This work investigated bacteriophage induced starter failures in artisanal buffalo Mozzarella production plants in Southern Italy. Two hundred and ten samples of whey starter cultures were screened for bacteriophage infection. Multiplex polymerase chain reaction (PCR) revealed phage infection in 28.56% of samples, all showing acidification problems during cheese making. Based on DNA sequences, bacteriophages for Lactococcus lactis (L. lactis), Lactobacillus delbruekii (L. delbruekii) and Streptococcus thermophilus (S. thermophilus) were detected. Two phages active against L.  lactis, фApr-1 and фApr-2, were isolated and characterised. The genomes, approximately 31.4 kb and 31 kb for фApr-1 and фApr-2 respectively, consisted of double-stranded linear DNA with pac-type system. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed one major structural protein of approximately 32.5 kDa and several minor proteins. This is the first report of phage isolation in buffalo milk and of the use of multiplex PCR to screen and study the diversity of phages against Lactic Acid Bacteria (LAB) strains in artisanal Water Buffalo Mozzarella starters. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale en
dc.relation.uri https://www.researchgate.net/profile/Giuseppe_Aprea2/publication/320551161_Multiplex_PCR_to_detect_bacteriophages_from_natural_whey_cultures_of_buffalo_milk_and_characterisation_of_two_phages_active_against_Lactococcus_lactis_phApr-1_and_phApr-2/links/5a156b984585150052133d8e/Multiplex-PCR-to-detect-bacteriophages-from-natural-whey-cultures-of-buffalo-milk-and-characterisation-of-two-phages-active-against-Lactococcus-lactis-phApr-1-and-phApr-2.pdf
dc.rights © 2017, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise. Open Access:This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.subject Bacteriophage en
dc.subject Buffalo en
dc.subject Lactic Acid Bacteria (LAB) en
dc.subject Lactococcus lactis en
dc.subject Milk en
dc.subject Natural starter culture en
dc.subject PCR en
dc.title Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother g.fitzgerald@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Veterinaria Italiana en
dc.internal.IRISemailaddress Gerald Fitzgerald, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: g.fitzgerald@ucc.ie en


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