A study of mRNA translation with computational analysis of ribosome profiling datasets

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dc.contributor.advisor Baranov, Pavel V. en
dc.contributor.author O'Connor, Patrick
dc.date.accessioned 2018-02-09T12:07:46Z
dc.date.available 2018-02-09T12:07:46Z
dc.date.issued 2017
dc.date.submitted 2017
dc.identifier.citation O'Connor, P. 2017. A study of mRNA translation with computational analysis of ribosome profiling datasets. PhD Thesis, University College Cork. en
dc.identifier.endpage 183 en
dc.identifier.uri http://hdl.handle.net/10468/5431
dc.description.abstract Ribosome profiling is based on capturing and sequencing of the mRNA fragments enclosed within the translating ribosome and it thereby provides a “snapshot” of ribosome positions at the transcriptome wide level. The approach was developed in 2009 and was a significant advancement towards the better understanding of the regulation of protein synthesis. I this thesis I describe my analysis of ribosome profiling data. In Chapter 1, I present a review of the recent developments of understanding obtained with ribosome profiling as well as discussing the implications of artifacts on the interpretation of its data. Chapters 2 and 3 details using ribosome profiling to examine the translational response to eIF2 repression and to the deprivation of oxygen and glucose respectively. Chapter 4 details how the interaction of the Shine Dalgarno with the ribosome rRNA alters the length of the mRNA protected fragments. In Chapter 5, I present analysis at identifying the relative impact of mRNA features on local ribosome profiling read density. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2017, Patrick O'Connor. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Translation en
dc.subject Ribosome profiling en
dc.title A study of mRNA translation with computational analysis of ribosome profiling datasets en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en
dc.check.info No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Science Foundation Ireland en
dc.description.status Not peer reviewed en
dc.internal.school Biochemistry en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
ucc.workflow.supervisor p.baranov@ucc.ie
dc.internal.conferring Spring 2018 en

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© 2017, Patrick O'Connor. Except where otherwise noted, this item's license is described as © 2017, Patrick O'Connor.
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