dc.contributor.advisor |
Baranov, Pavel V. |
en |
dc.contributor.author |
O'Connor, Patrick |
|
dc.date.accessioned |
2018-02-09T12:07:46Z |
|
dc.date.available |
2018-02-09T12:07:46Z |
|
dc.date.issued |
2017 |
|
dc.date.submitted |
2017 |
|
dc.identifier.citation |
O'Connor, P. 2017. A study of mRNA translation with computational analysis of ribosome profiling datasets. PhD Thesis, University College Cork. |
en |
dc.identifier.endpage |
183 |
en |
dc.identifier.uri |
http://hdl.handle.net/10468/5431 |
|
dc.description.abstract |
Ribosome profiling is based on capturing and sequencing of the mRNA fragments enclosed within the translating ribosome and it thereby provides a “snapshot” of ribosome positions at the transcriptome wide level. The approach was developed in 2009 and was a significant advancement towards the better understanding of the regulation of protein synthesis. I this thesis I describe my analysis of ribosome profiling data. In Chapter 1, I present a review of the recent developments of understanding obtained with ribosome profiling as well as discussing the implications of artifacts on the interpretation of its data. Chapters 2 and 3 details using ribosome profiling to examine the translational response to eIF2 repression and to the deprivation of oxygen and glucose respectively. Chapter 4 details how the interaction of the Shine Dalgarno with the ribosome rRNA alters the length of the mRNA protected fragments. In Chapter 5, I present analysis at identifying the relative impact of mRNA features on local ribosome profiling read density. |
en |
dc.format.mimetype |
application/pdf |
en |
dc.language.iso |
en |
en |
dc.publisher |
University College Cork |
en |
dc.rights |
© 2017, Patrick O'Connor. |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
en |
dc.subject |
Translation |
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dc.subject |
Ribosome profiling |
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dc.title |
A study of mRNA translation with computational analysis of ribosome profiling datasets |
en |
dc.type |
Doctoral thesis |
en |
dc.type.qualificationlevel |
Doctoral |
en |
dc.type.qualificationname |
PhD (Science) |
en |
dc.internal.availability |
Full text available |
en |
dc.check.info |
No embargo required |
en |
dc.description.version |
Accepted Version |
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dc.contributor.funder |
Science Foundation Ireland
|
en |
dc.description.status |
Not peer reviewed |
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dc.internal.school |
Biochemistry |
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dc.check.type |
No Embargo Required |
|
dc.check.reason |
No embargo required |
en |
dc.check.opt-out |
Not applicable |
en |
dc.thesis.opt-out |
false |
|
dc.check.embargoformat |
Not applicable |
en |
ucc.workflow.supervisor |
p.baranov@ucc.ie |
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dc.internal.conferring |
Spring 2018 |
en |