Faecal microRNAs: indicators of imbalance at the host-microbe interface?

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dc.contributor.author Moloney, Gerard M.
dc.contributor.author Viola, Maria F.
dc.contributor.author Hoban, Alan E.
dc.contributor.author Dinan, Timothy G.
dc.contributor.author Cryan, John F.
dc.date.accessioned 2018-02-16T12:33:49Z
dc.date.available 2018-02-16T12:33:49Z
dc.date.issued 2017-12-21
dc.identifier.citation Moloney, G. M., Viola, M. F., Hoban, A. E., Dinan, T. G. and Cryan, J. F. 'Faecal microRNAs: indicators of imbalance at the host-microbe interface?', Beneficial Microbes, In Press, pp. 1-10. doi: 10.3920/bm2017.0013 en
dc.identifier.volume 0 en
dc.identifier.issued 0 en
dc.identifier.startpage 1 en
dc.identifier.endpage 10 en
dc.identifier.issn 1876-2883; 1876-2891
dc.identifier.uri http://hdl.handle.net/10468/5470
dc.identifier.doi 10.3920/bm2017.0013
dc.description.abstract The enteric microbiota is characterised by a balance and composition that is unique to the host. It is important to understand the mechanisms through which the host can maintain the composition of the gut microbiota. MicroRNAs (miRNA) are implicated in intercellular communication and have been isolated from bodily fluids including stool. Recent findings suggest that miRNA produced by the host’s intestinal epithelial cells (IECs) participate in shaping the microbiota. To investigate whether miRNA expression was influenced by the gut microbiota we measured the expression of miRNAs expressed by intestinal epithelial cells in faeces. Specifically, we measured miRNA expression in faeces from germ-free (GF) and conventional mice and similarly in a rat model of antibiotic-mediated depletion of the gut microbiota control rats. In adult male GF and conventional mice and adult Sprague Dawley (SD) rats were treated with a combination of antibiotics for 8 weeks; total RNA was extracted from faecal pellets taken at week 0, 2, 4, 6 week 8 and the expression of let-7b-3p, miR-141-3p, miR-200a-3p and miR-1224-5p (miRNAs known to be expressed in IECs) were measured relative to U6 at each time point using qRT-PCR. In GF animals the expression of let-7b, miR-141 and miR-200a in faeces was lower compared to conventional mice. Following antibiotic-mediated depletion of gut microbiota, rats showed two divergent profiles of miRNA expression. Following two weeks of antibiotic treatment, the expression of let-7b and miR-1224 dropped significantly and remained low for the remainder of the study. The expression of miR-200a and miR-141 was significantly higher at week 2 than before antibiotic treatment commenced. Subsequently, the expression of miR-200a and miR-141 decreased at week 4 and continued to decrease at week 6. This data demonstrates that miRNAs can be used as an independent, non-invasive marker of microbial fluctuations along with gut pathology in the intestine. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Wageningen Academic Publishers en
dc.relation.uri http://www.wageningenacademic.com/doi/abs/10.3920/BM2017.0013
dc.rights © 2017 Wageningen Academic Publishers. Published under a Creative Commons license https://creativecommons.org/licenses/by-nc-sa/4.0/ en
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/4.0/ en
dc.subject Microbiota en
dc.subject MicroRNA en
dc.subject Enteric en
dc.subject Stool en
dc.subject Host en
dc.title Faecal microRNAs: indicators of imbalance at the host-microbe interface? en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John F Cryan, Department Of Anatomy & Neuroscience, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.cryan@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2018-02-16T12:24:08Z
dc.description.version Published Version en
dc.internal.rssid 426109647
dc.description.status Peer reviewed en
dc.identifier.journaltitle Beneficial microbes en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress J.Cryan@ucc.ie en
dc.internal.bibliocheck In Press Feb 2018. Update citation, vol, page numbers etc. If needed replace article with final published version if this also has a cc license en


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© 2017 Wageningen Academic Publishers. Published under a Creative Commons license https://creativecommons.org/licenses/by-nc-sa/4.0/ Except where otherwise noted, this item's license is described as © 2017 Wageningen Academic Publishers. Published under a Creative Commons license https://creativecommons.org/licenses/by-nc-sa/4.0/
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