In vitro cytotoxicity of water soluble silver (Ag) nanoparticles on HaCat and A549 cell lines

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dc.contributor.author Garvey, Mary
dc.contributor.author Padmanabhan, Sibu C.
dc.contributor.author Pillai, Suresh C.
dc.contributor.author Cruz-Romero, Malco
dc.contributor.author Kerry, Joseph P.
dc.contributor.author Morris, Michael A.
dc.date.accessioned 2018-02-20T13:24:13Z
dc.date.available 2018-02-20T13:24:13Z
dc.date.issued 2017
dc.identifier.citation Garvey, M., Padmanabhan, S. and Pillai, S. (2017) 'In vitro cytotoxicity of water soluble silver (Ag) nanoparticles on HaCat and A549 cell lines', Journal of Toxicology and Pharmacology, 1(3), 016 (8pp) en
dc.identifier.volume 1
dc.identifier.issued 3
dc.identifier.startpage 1
dc.identifier.endpage 8
dc.identifier.uri http://hdl.handle.net/10468/5508
dc.description.abstract The wide range of applications of silver nanoparticles (AgNPs) in commercial products, including food packaging, has encouraged researchers to come up with novel preparation methods for the production of these robust materials. The methods resulting in the formation of NPs for such commercial applications clearly demand a good accounting of their toxicity aspects to humans as well as the environment. We herein present a chemical preparation method for the production of size- and shape-defined AgNPs and investigate the impact of these nanoparticles on HaCat and A549 cell lines. Findings show that lung cells (A549) are more sensitive than skin cells (HaCat) to Ag induced toxicity, evident by the significantly (p<0.05) reduced LC50 for all NPs under study. The current investigation showed that the extent of surface capping agent (citrate) and size influenced the cell toxicity, where a lesser surface coverage (zeta potential, ζ, -27.7 mV) and smaller size (~17 nm) enhanced the toxicity compared to comparatively bigger particles (~39 nm) with higher surface coverage (ζ, -47.3 mV). The size- and shape-defined particles such as triangles which have proven useful for many applications, due to their high energy/high field edges, were found to be less toxic against biological cell lines and therefore may have potential to be used in food packaging applications as reservoirs of silver ions. A striking difference in cell line toxicity within such a small size window clearly demonstrates the vital roles played by the smaller size, difference in shape and lesser surface coverage in defining a higher passive cell membrane diffusion followed by silver dissolution inside cell cytoplasm increasing cytotoxicity. en
dc.description.sponsorship Enterprise Ireland (Commercialisation Fund (CF/2014/4370); Department of Agriculture, Food and the Marine (Food Institutional Research Measure (FIRM)11/F/038 ) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Scientific Open Access Journals en
dc.relation.uri http://www.scientificoajournals.org/jtp.016.php
dc.rights © 2017, the Authors. Published by Scientific Open Access Journals LLC. Works published by the Scientific Open Access Journals are under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited; the original copy writes are retained to the author. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Silver nanoparticles en
dc.subject Cytotoxicity en
dc.subject Lung cell en
dc.subject Skin cells en
dc.title In vitro cytotoxicity of water soluble silver (Ag) nanoparticles on HaCat and A549 cell lines en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Sibu C Padmanabhan, Chemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: sc.padmanabhan@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Department of Agriculture, Food and the Marine
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Enterprise Ireland
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Toxicology and Pharmacology en
dc.internal.IRISemailaddress sc.padmanabhan@ucc.ie en
dc.internal.IRISemailaddress m.cruz@ucc.ie en
dc.identifier.articleid 16
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2278/IE/Advanced Materials and BioEngineering Research Centre (AMBER)/


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© 2017, the Authors. Published by Scientific Open Access Journals LLC. Works published by the Scientific Open Access Journals are under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited; the original copy writes are retained to the author. Except where otherwise noted, this item's license is described as © 2017, the Authors. Published by Scientific Open Access Journals LLC. Works published by the Scientific Open Access Journals are under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited; the original copy writes are retained to the author.
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