Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei

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dc.contributor.author Ojha, K. Shikha
dc.contributor.author Burgess, Catherine M.
dc.contributor.author Duffy, Geraldine
dc.contributor.author Kerry, Joseph P.
dc.contributor.author Tiwari, Brijesh K.
dc.date.accessioned 2018-02-20T13:24:14Z
dc.date.available 2018-02-20T13:24:14Z
dc.date.issued 2018
dc.identifier.citation Ojha, K. S., Burgess, C. M., Duffy, G., Kerry, J. P. and Tiwari, B. K. (2018) 'Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei', PLOS ONE, 13(1), e0191053 (20pp). doi: 10.1371/journal.pone.0191053 en
dc.identifier.volume 13
dc.identifier.issued 1
dc.identifier.startpage 1
dc.identifier.endpage 20
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/5516
dc.identifier.doi 10.1371/journal.pone.0191053
dc.description.abstract The lethal effects of soundwaves on a range of microorganisms have been known for almost a century whereas, the use of ultrasound to promote or control their activity is much more recent. Moreover, the fundamental molecular mechanism influencing the behaviour of microorganisms subjected to ultrasonic waves is not well established. In this study, we investigated the influence of ultrasonic frequencies of 20, 45, 130 and 950 kHz on growth kinetics of Lactobacillus sakei. A significant increase in the growth rate of L. sakei was observed following ultrasound treatment at 20 kHz despite the treatment yielding a significant reduction of ca. 3 log cfu/mL in cells count. Scanning electron microscopy showed that ultrasound caused significant changes on the cell surface of L. sakei culture with the formation of pores “sonoporation”. Phenotypic microarrays showed that all ultrasound treated L. sakei after exposure to various carbon, nitrogen, phosphorus and sulphur sources had significant variations in nutrient utilisation. Integration of this phenotypic data with the genome of L. sakei revealed that various metabolic pathways were being influenced by the ultrasound treatments. Results presented in this study showed that the physiological response of L. sakei in response to US is frequency dependent and that it can influence metabolic pathways. Hence, ultrasound treatments can be employed to modulate microbial activity for specialised applications. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.relation.uri http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191053
dc.rights © 2018, Ojha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Ribose en
dc.subject Metabolic pathways en
dc.subject Genomics en
dc.subject Nitrogen metabolism en
dc.subject Sulfur en
dc.subject Cell membranes en
dc.subject Sound pressure en
dc.subject Pentoses en
dc.title Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Joseph Kerry, Food & Nutritional Sciences, University College Cork, Cork, Ireland. +353-21-490-3000 Email: joe.kerry@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Teagasc
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress joe.kerry@ucc.ie en
dc.identifier.articleid e0191053


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© 2018, Ojha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2018, Ojha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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