Rhinoscleroma pathogenesis: the type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formation

Show simple item record

dc.contributor.author Corelli, Barbara
dc.contributor.author Almeida, Ana S.
dc.contributor.author Sonego, Fabiane
dc.contributor.author Castiglia, Virginia
dc.contributor.author Fevre, Cindy
dc.contributor.author Brisse, Sylvain
dc.contributor.author Sansonetti, Philippe J.
dc.contributor.author Tournebize, Régis
dc.date.accessioned 2018-03-09T12:55:07Z
dc.date.available 2018-03-09T12:55:07Z
dc.date.issued 2018
dc.identifier.citation Corelli, B., Almeida, A. S., Sonego, F., Castiglia, V., Fevre, C., Brisse, S., Sansonetti, P. J. and Tournebize, R. (2018) 'Rhinoscleroma pathogenesis: the type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formation', PLOS Neglected Tropical Diseases, 12(1), e0006201 (19pp). doi: 10.1371/journal.pntd.0006201 en
dc.identifier.volume 12
dc.identifier.issued 1
dc.identifier.startpage 1
dc.identifier.endpage 19
dc.identifier.issn 1935-2727
dc.identifier.issn 1935-2735
dc.identifier.uri http://hdl.handle.net/10468/5610
dc.identifier.doi 10.1371/journal.pntd.0006201
dc.description.abstract Rhinoscleroma is a human specific chronic granulomatous infection of the nose and upper airways caused by the Gram-negative bacterium Klebsiella pneumoniae subsp. rhinoscleromatis. Although considered a rare disease, it is endemic in low-income countries where hygienic conditions are poor. A hallmark of this pathology is the appearance of atypical foamy monocytes called Mikulicz cells. However, the pathogenesis of rhinoscleroma remains poorly investigated. Capsule polysaccharide (CPS) is a prominent virulence factor in bacteria. All K. rhinoscleromatis strains are of K3 serotype, suggesting that CPS can be an important driver of rhinoscleroma disease. In this study, we describe the creation of the first mutant of K. rhinoscleromatis, inactivated in its capsule export machinery. Using a murine model recapitulating the formation of Mikulicz cells in lungs, we observed that a K. rhinoscleromatis CPS mutant (KR cps-) is strongly attenuated and that mice infected with a high dose of KR cps- are still able to induce Mikulicz cells formation, unlike a K. pneumoniae capsule mutant, and to partially recapitulate the characteristic strong production of IL-10. Altogether, the results of this study show that CPS is a virulence factor of K. rhinoscleromatis not involved in the specific appearance of Mikulicz cells. en
dc.description.sponsorship Fundaçao para a Ciência e a Tecnologia (Investissement d'Avenir program Laboratoire d'Excellence "Integrative Biology of Emerging Infectious Diseases" ( ANR-10-LABX-62-IBEID); Agence Nationale de la Recherche (Infrastructure d’Avenir en Biologie et Santé "France Life Imaging" (ANR-11-INBS-0006); France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.relation.uri http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006201
dc.rights © 2018, Corelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Rhinoscleroma en
dc.subject Mikulicz cells en
dc.subject Infection en
dc.subject Lungs en
dc.title Rhinoscleroma pathogenesis: the type K3 capsule of Klebsiella rhinoscleromatis is a virulence factor not involved in Mikulicz cells formation en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Ana Almeida, APC Microbiome Institute, University College Cork, Cork, Ireland. +353 214904380. Email: a.almeida@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Seventh Framework Programme
dc.contributor.funder Fondation pour la Recherche Médicale
dc.contributor.funder Fundação para a Ciência e a Tecnologia
dc.contributor.funder Agence Nationale de la Recherche
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS Neglected Tropical Diseases en
dc.identifier.articleid e0006201
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/316682/EU/Infection biology training network: shaping the future of infectious diseases treatments/INBIONET


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2018, Corelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2018, Corelli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement