Bifidobacterium breve UCC2003 employs multiple transcriptional regulators to control metabolism of particular human milk oligosaccharides

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dc.contributor.author James, Kieran
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Penno, Christophe
dc.contributor.author O'Brien, Rebecca L.
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2018-03-22T09:25:50Z
dc.date.available 2018-03-22T09:25:50Z
dc.date.issued 2018-03-02
dc.identifier.citation James, K., O'Connell Motherway, M., Penno, C., O'Brien, R. L. and van Sinderen, D. (2018) 'Bifidobacterium breve UCC2003 employs multiple transcriptional regulators to control metabolism of particular human milk oligosaccharides', Applied and Environmental Microbiology. doi:10.1128/aem.02774-17 en
dc.identifier.issn 0099-2240
dc.identifier.issn 1098-5336
dc.identifier.uri http://hdl.handle.net/10468/5673
dc.identifier.doi 10.1128/aem.02774-17
dc.description.abstract Bifidobacterial carbohydrate metabolism has been studied in considerable detail for a variety of both plant and human-derived glycans, particularly involving the bifidobacterial prototype Bifidobacterium breve UCC2003. We recently elucidated the metabolic pathways by which the human milk oligosaccharide (HMO) constituents lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT) and lacto-N-biose (LNB) are utilized by B. breve UCC2003. However, to date no work has been carried out on the regulatory mechanisms that control expression of the genetic loci involved in these HMO metabolic pathways. In the current study, we describe the characterization of three transcriptional regulators and corresponding operator and associated (inducible) promoter sequences, the latter governing transcription of the genetic elements involved in LN(n)T/LNB metabolism. The activity of these regulators is dependent on the release of specific monosaccharides, which are believed to act as allosteric effectors, and which are derived from the corresponding HMOs targeted by the particular locus.Importance Human milk oligosaccharides (HMOs) are a key factor in the development of the breastfed infant microbiota. They function as prebiotics, selecting for a specific range of microbes, including a number of infant-associated species of bifidobacteria, which are thought to provide a range of health benefits to the infant host. While much research has been carried out on elucidating the mechanisms of HMO metabolism in infant-associated bifidobacteria, there is to date very little understanding of the transcriptional regulation of these pathways. The current study reveals a multi-component transcriptional regulation system that controls the recently-identified pathways of HMO metabolism in the infant-associated Bifidobacterium breve prototype strain UCC2003. This not only provides insight into the regulatory mechanisms present in other infant-associated bifidobacteria, but also provides an example of a network of sequential steps regulating microbial carbohydrate metabolism. en
dc.description.sponsorship Irish Research Council (Postgraduate Research Project Award GOIPG/2013/651); Health Research Board (Grant No. PDTM/20011/9) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.rights © 2018, American Society for Microbiology. All Rights Reserved. en
dc.subject Bifidobacteria en
dc.subject Probiotic en
dc.subject Prebiotic en
dc.subject Transcriptional regulation en
dc.subject HMO en
dc.subject Carbohydrate metabolism en
dc.title Bifidobacterium breve UCC2003 employs multiple transcriptional regulators to control metabolism of particular human milk oligosaccharides en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2018-03-12T10:10:08Z
dc.description.version Accepted Version en
dc.internal.rssid 429271301
dc.contributor.funder Irish Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Health Research Board en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Applied and Environmental Microbiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.internal.bibliocheck In Press. Check for vol. / issue / page numbers / supplementary files. Amend citation as necessary.
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/ en


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