Transcriptional slippage in the positive-sense RNA virus family Potyviridae

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dc.contributor.author Olspert, Allan
dc.contributor.author Chung, Betty Y-W
dc.contributor.author Atkins, John F.
dc.contributor.author Carr, John P.
dc.contributor.author Firth, Andrew E.
dc.date.accessioned 2018-03-29T11:03:45Z
dc.date.available 2018-03-29T11:03:45Z
dc.date.issued 2015-06-25
dc.identifier.citation Olspert, A., Chung, B. Y. W., Atkins, J. F., Carr, J. P. and Firth, A. E. (2015) 'Transcriptional slippage in the positive‐sense RNA virus family Potyviridae', EMBO reports, 16(8), pp. 995-1004. doi: 10.15252/embr.201540509 en
dc.identifier.volume 16 en
dc.identifier.issued 8 en
dc.identifier.startpage 995 en
dc.identifier.endpage 1004 en
dc.identifier.issn 1469-3178
dc.identifier.uri http://hdl.handle.net/10468/5718
dc.identifier.doi 10.15252/embr.201540509
dc.description.abstract The family Potyviridae encompasses ~30% of plant viruses and is responsible for significant economic losses worldwide. Recently, a small overlapping coding sequence, termed pipo, was found to be conserved in the genomes of all potyvirids. PIPO is expressed as part of a frameshift protein, P3N‐PIPO, which is essential for virus cell‐to‐cell movement. However, the frameshift expression mechanism has hitherto remained unknown. Here, we demonstrate that transcriptional slippage, specific to the viral RNA polymerase, results in a population of transcripts with an additional “A” inserted within a highly conserved GAAAAAA sequence, thus enabling expression of P3N‐PIPO. The slippage efficiency is ~2% in Turnip mosaic virus and slippage is inhibited by mutations in the GAAAAAA sequence. While utilization of transcriptional slippage is well known in negative‐sense RNA viruses such as Ebola, mumps and measles, to our knowledge this is the first report of its widespread utilization for gene expression in positive‐sense RNA viruses. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher EMBO Press en
dc.relation.uri http://embor.embopress.org/content/embor/16/8/995.full.pdf
dc.rights © 2015 The Authors. Published by EMBO Press under the terms of the CC BY 4.0 license en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Gene expression en
dc.subject P3N-PIPO en
dc.subject Potyvirus en
dc.subject RNA virus en
dc.subject Transcriptional slippage en
dc.title Transcriptional slippage in the positive-sense RNA virus family Potyviridae en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John F. Atkins, Biochemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.atkins@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2018-03-29T10:49:27Z
dc.description.version Published Version en
dc.internal.rssid 431799741
dc.contributor.funder Wellcome Trust en
dc.contributor.funder Biotechnology and Biological Sciences Research Council en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Embo Reports en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress j.atkins@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/WT/Immunology and Infectious Disease/088789//Systematic Bioinformatic Discovery of 'Hidden' Virus Genes./ en
dc.relation.project info:eu-repo/grantAgreement/WT/Immunology and Infectious Disease/106207//Encyclopedia of RNA Virus Elements: systematic bioinformatic discovery and experimental analysis of hidden genes, non-canonical translation mechanisms, and other functional elements in RNA virus genomes./ en
dc.relation.project info:eu-repo/grantAgreement/RCUK/BBSRC/BB/J007072/1/GB/Non-canonical gene expression: Investigating a novel stimulator and a novel function for ribosomal frameshifting/ en
dc.relation.project info:eu-repo/grantAgreement/RCUK/BBSRC/BB/J015652/1/GB/Deciphering the enigmatic expression mechanism of the newly discovered PIPO gene in the Potyviridae family of plant viruses/ en
dc.relation.project info:eu-repo/grantAgreement/RCUK/BBSRC/BB/J015652/1/GB/Deciphering the enigmatic expression mechanism of the newly discovered PIPO gene in the Potyviridae family of plant viruses/ en
dc.relation.project info:eu-repo/grantAgreement/RCUK/BBSRC/BB/J011762/1/GB/Modelling and manipulation of plant-aphid interactions: A new avenue for sustainable disease management of an important crop in Africa/ en


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© 2015 The Authors. Published by EMBO Press under the terms of the CC BY 4.0 license Except where otherwise noted, this item's license is described as © 2015 The Authors. Published by EMBO Press under the terms of the CC BY 4.0 license
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