Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates

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dc.contributor.author Ortiz-Merino, Raúl A.
dc.contributor.author Varela, Javier A.
dc.contributor.author Coughlan, Aisling Y.
dc.contributor.author Hoshida, Hisashi
dc.contributor.author da Silveira, Wendel B.
dc.contributor.author Wilde, Caroline
dc.contributor.author Kuijpers, Niels G. A.
dc.contributor.author Geertman, Jan-Maarten
dc.contributor.author Wolfe, Kenneth H.
dc.contributor.author Morrissey, John P.
dc.date.accessioned 2018-05-02T10:16:22Z
dc.date.available 2018-05-02T10:16:22Z
dc.date.issued 2018
dc.identifier.citation Ortiz-Merino, R. A., Varela, J. A., Coughlan, A. Y., Hoshida, H., da Silveira, W. B., Wilde, C., Kuijpers, N. G. A., Geertman, J.-M., Wolfe, K. H. and Morrissey, J. P. (2018) 'Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates', Frontiers in Genetics, 9, 94 (16pp). doi: 10.3389/fgene.2018.00094 en
dc.identifier.volume 9
dc.identifier.issn 1664-8021
dc.identifier.uri http://hdl.handle.net/10468/5930
dc.identifier.doi 10.3389/fgene.2018.00094
dc.description.abstract Kluyveromyces marxianus is traditionally associated with fermented dairy products, but can also be isolated from diverse non-dairy environments. Because of thermotolerance, rapid growth and other traits, many different strains are being developed for food and industrial applications but there is, as yet, little understanding of the genetic diversity or population genetics of this species. K. marxianus shows a high level of phenotypic variation but the only phenotype that has been clearly linked to a genetic polymorphism is lactose utilisation, which is controlled by variation in the LAC12 gene. The genomes of several strains have been sequenced in recent years and, in this study, we sequenced a further nine strains fromdifferent origins. Analysis of the Single Nucleotide Polymorphisms (SNPs) in 14 strains was carried out to examine genome structure and genetic diversity. SNP diversity in K. marxianus is relatively high, with up to 3% DNA sequence divergence between alleles. It was found that the isolates include haploid, diploid, and triploid strains, as shown by both SNP analysis and flow cytometry. Diploids and triploids contain long genomic tracts showing loss of heterozygosity (LOH). All six isolates from dairy environments were diploid or triploid, whereas 6 out 7 isolates from non-dairy environment were haploid. This also correlated with the presence of functional LAC12 alleles only in dairy haplotypes. The diploids were hybrids between a non-dairy and a dairy haplotype, whereas triploids included three copies of a dairy haplotype. en
dc.description.sponsorship Consejo Nacional de Ciencia y Tecnología (fellowship number 440667); Japan Science and Technology Agency (Adaptable and the Advanced Low Carbon Technology R&D Program) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media SA en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fgene.2018.00094/full
dc.rights © 2018, Ortiz-Merino, Varela, Coughlan, Hoshida, da Silveira, Wilde, Kuipers, Geertman, Wolfe and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Lactose transport en
dc.subject Non-conventional yeast en
dc.subject Yeast evolution en
dc.subject Industrial yeast en
dc.subject Dairy en
dc.subject Kluyveromyces en
dc.subject Lac12 en
dc.title Ploidy variation in Kluyveromyces marxianus separates dairy and non-dairy isolates en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John Morrissey, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: J.Morrissey@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Seventh Framework Programme
dc.contributor.funder Consejo Nacional de Ciencia y Tecnología
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Japan Science and Technology Agency
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Genetics en
dc.internal.IRISemailaddress j.morrissey@ucc.ie en
dc.identifier.articleid 94
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/606795/EU/Yeast Cell Factories: Training Researchers to Apply Modern Post-Genomic Methods In Yeast Biotechnology/YEASTCELL
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1910/IE/Sexual cycles, genomics, and mating-type switching in non-conventional yeast species/


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© 2018, Ortiz-Merino, Varela, Coughlan, Hoshida, da Silveira, Wilde, Kuipers, Geertman, Wolfe and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2018, Ortiz-Merino, Varela, Coughlan, Hoshida, da Silveira, Wilde, Kuipers, Geertman, Wolfe and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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