Pasture feeding changes the bovine rumen and milk metabolome

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dc.contributor.author O'Callaghan, Tom F.
dc.contributor.author Vázquez-Fresno, Rosa
dc.contributor.author Serra-Cayuela, Arnau
dc.contributor.author Dong, Edison
dc.contributor.author Mandal, Rupasri
dc.contributor.author Hennessy, Deirdre
dc.contributor.author McAuliffe, Stephen
dc.contributor.author Dillon, Pat
dc.contributor.author Wishart, David
dc.contributor.author Stanton, Catherine
dc.contributor.author Ross, R. Paul
dc.date.accessioned 2018-05-02T10:16:31Z
dc.date.available 2018-05-02T10:16:31Z
dc.date.issued 2018
dc.identifier.citation O’Callaghan, T., Vázquez-Fresno, R., Serra-Cayuela, A., Dong, E., Mandal, R., Hennessy, D., McAuliffe, S., Dillon, P., Wishart, D., Stanton, C. and Ross, R. (2018) 'Pasture feeding changes the bovine rumen and milk metabolome', Metabolites, 8(2), 27 (24pp). doi: 10.3390/metabo8020027 en
dc.identifier.volume 8
dc.identifier.issued 2
dc.identifier.startpage 1
dc.identifier.endpage 24
dc.identifier.issn 2218-1989
dc.identifier.uri http://hdl.handle.net/10468/5972
dc.identifier.doi 10.3390/metabo8020027
dc.description.abstract The purpose of this study was to examine the effects of two pasture feeding systems—perennial ryegrass (GRS) and perennial ryegrass and white clover (CLV)—and an indoor total mixed ration (TMR) system on the (a) rumen microbiome; (b) rumen fluid and milk metabolome; and (c) to assess the potential to distinguish milk from different feeding systems by their respective metabolomes. Rumen fluid was collected from nine rumen cannulated cows under the different feeding systems in early, mid and late lactation, and raw milk samples were collected from ten non-cannulated cows in mid-lactation from each of the feeding systems. The microbiota present in rumen liquid and solid portions were analysed using 16S rRNA gene sequencing, while 1H-NMR untargeted metabolomic analysis was performed on rumen fluid and raw milk samples. The rumen microbiota composition was not found to be significantly altered by any feeding system in this study, likely as a result of a shortened adaptation period (two weeks’ exposure time). In contrast, feeding system had a significant effect on both the rumen and milk metabolome. Increased concentrations of volatile fatty acids including acetic acid, an important source of energy for the cow, were detected in the rumen of TMR and CLV-fed cows. Pasture feeding resulted in significantly higher concentrations of isoacids in the rumen. The ruminal fluids of both CLV and GRS-fed cows were found to have increased concentrations of p-cresol, a product of microbiome metabolism. CLV feeding resulted in increased rumen concentrations of formate, a substrate compound for methanogenesis. The TMR feeding resulted in significantly higher rumen choline content, which contributes to animal health and milk production, and succinate, a product of carbohydrate metabolism. Milk and rumen-fluids were shown to have varying levels of dimethyl sulfone in each feeding system, which was found to be an important compound for distinguishing between the diets. CLV feeding resulted in increased concentrations of milk urea. Milk from pasture-based feeding systems was shown to have significantly higher concentrations of hippuric acid, a potential biomarker of pasture-derived milk. This study has demonstrated that 1H-NMR metabolomics coupled with multivariate analysis is capable of distinguishing both rumen-fluid and milk derived from cows on different feeding systems, specifically between indoor TMR and pasture-based diets used in this study. en
dc.description.sponsorship Dairy Research Ireland (Dairy Levy Fund) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI AG en
dc.relation.uri http://www.mdpi.com/2218-1989/8/2/27
dc.rights © 2018, the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).© 2018 by the authors. Licensee MDPI, Basel, Switzerland. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Cows diet en
dc.subject Rumen en
dc.subject Milk en
dc.subject Metabolome en
dc.subject Pasture en
dc.subject Total mixed ration en
dc.title Pasture feeding changes the bovine rumen and milk metabolome en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul Ross, APC Microbiome Institute, University College Cork, Cork, Ireland. +353-21-490-3000 E-mail:p.ross@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Dairy Research Ireland
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Teagasc
dc.description.status Peer reviewed en
dc.identifier.journaltitle Metabolites en
dc.internal.IRISemailaddress p.ross@ucc.ie en
dc.identifier.articleid 27
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/


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© 2018, the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Except where otherwise noted, this item's license is described as © 2018, the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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