Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: a redox proteomic investigation

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dc.contributor.author Bouallegui, Younes
dc.contributor.author Ben Younes, Ridha
dc.contributor.author Oueslati, Ridha
dc.contributor.author Sheehan, David
dc.date.accessioned 2018-05-15T10:43:59Z
dc.date.available 2018-05-15T10:43:59Z
dc.date.issued 2018-04-23
dc.identifier.citation Bouallegui, Y., Ben Younes, R., Oueslati, R. and Sheehan, D. (2018) 'Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: a redox proteomic investigation', Aquatic Toxicology. doi: 10.1016/j.aquatox.2018.04.013 en
dc.identifier.issn 0166-445X
dc.identifier.uri http://hdl.handle.net/10468/6103
dc.identifier.doi 10.1016/j.aquatox.2018.04.013
dc.description.abstract Oxidative stress is often implicated in nanoparticle toxicity. Several studies have highlighted the role of internalization routes in determining nanotoxicity. Here, we investigate how two endocytotic mechanisms (clathrin- and caveolae-mediated) impact on redox balance in gill and digestive gland of the mussel, Mytilus galloprovincialis. Animals were exposed (for 3, 6 and 12 h) to two sizes of silver nanoparticles (AgNP: <50 nm and <100 nm) prior to and after blockade of two endocytic pathways (amantadine blocks clathrin-mediated endocytosis while nystatin blocks caveolae-mediated endocytosis). Redox-proteomic tools were used to determine effects. Our results demonstrate the ability of both sizes of AgNP (<50 and <100 nm) to cause protein thiol oxidation and/or protein carbonylation. However, blockade of endocytotic routes mitigated AgNP toxicity. Differential ROS-related toxicity of AgNP to mussel tissues seemed to be linked to tissue-specific mode of action requirements. Cell uptake mechanism strongly influences toxicity of AgNPs in this filter-feeder. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier B.V. en
dc.rights © 2018, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Oxidative stress en
dc.subject Carbonyls en
dc.subject Thiols en
dc.subject AgNPs en
dc.subject Endocytosis en
dc.subject Mussel en
dc.title Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: a redox proteomic investigation en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother David Sheehan, School Of Biochemistry & Cell Biology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.sheehan@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 24 months after publication by request of the publisher. en
dc.check.date 2020-04-23
dc.date.updated 2018-05-01T08:53:04Z
dc.description.version Accepted Version en
dc.internal.rssid 435972511
dc.contributor.funder Ministry of Higher Education and Scientific Research, Tunisia
dc.description.status Peer reviewed en
dc.identifier.journaltitle Aquatic Toxicology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.sheehan@ucc.ie en
dc.internal.bibliocheck In Press. Check for vol. / issue / page numbers / supplementary files. Amend citation as necessary.


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© 2018, Elsevier B.V. All rights reserved.  This manuscript version is made available under the CC-BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2018, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
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