Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains
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Supplementary Information
Supplementary Information
Date
2017-04-18
Authors
Varela, Javier A.
Montini, Noemi
Scully, Damhan
Van der Ploeg, Ralph
Oreb, Mislav
Boles, Eckhard
Hirota, Junya
Akada, Rinji
Hoshida, Hisashi
Morrissey, John P.
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press on behalf of Federation of European Microbiological Societies
Published Version
Abstract
Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transport via a permease and intracellular hydrolysis of the disaccharide. Given the importance of the trait, it was intriguing that most, but not all, strains of K. marxianus are reported to consume lactose efficiently. In this study, primarily through heterologous expression in S. cerevisiae and K. marxianus, it was established that a single gene, LAC12, is responsible for lactose uptake in K. marxianus. Strains that failed to transport lactose showed variation in 13 amino acids in the Lac12p protein, rendering the protein non-functional for lactose transport. Genome analysis showed that the LAC12 gene is present in four copies in the subtelomeric regions of three different chromosomes but only the ancestral LAC12 gene encodes a functional lactose transporter. Other copies of LAC12 may be non-functional or have alternative substrates. The analysis raises some interesting questions regarding the evolution of sugar transporters in K. marxianus.
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Keywords
Lactose utilisation , LAC12 , Kluyveromyces marxianus
Citation
Varela, J. A., Montini, N., Scully, D., Van der Ploeg, R., Oreb, M., Boles, E., Hirota, J., Akada, R., Hoshida, H. and Morrissey, J. P. (2017) 'Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains', FEMS Yeast Research, 17(3), fox021 (13pp). doi:10.1093/femsyr/fox021
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© 2017, FEMS. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS Yeast Research following peer review. The version of record Varela, J. A., Montini, N., Scully, D., Van der Ploeg, R., Oreb, M., Boles, E., Hirota, J., Akada, R., Hoshida, H. and Morrissey, J. P. (2017) 'Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains', FEMS Yeast Research, 17(3), fox021 (13pp). doi:10.1093/femsyr/fox021] is available online at: https://doi.org/10.1093/femsyr/fox021