HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters

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dc.contributor.author Choudhary, Alka
dc.contributor.author Naughton, Lynn M.
dc.contributor.author Dobson, Alan D. W.
dc.contributor.author Rai, Dilip K.
dc.date.accessioned 2018-07-11T10:37:42Z
dc.date.available 2018-07-11T10:37:42Z
dc.date.issued 2018-07-03
dc.identifier.citation Choudhary, A., Naughton, L. M., Dobson, A. D.W. and Rai, D. K. (2018) 'HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters', Rapid Communications in Mass Spectrometry. doi:10.1002/rcm.8226 en
dc.identifier.issn 0951-4198
dc.identifier.issn 1097-0231
dc.identifier.uri http://hdl.handle.net/10468/6451
dc.identifier.doi 10.1002/rcm.8226
dc.description.abstract Rationale: In recent years, metabolites produced by Pseudovibrio species have gained scientific attention due to their potent antimicrobial activity. Recently we also have assessed antibacterial activities of Pseudovibrio sp. W64 isolates against Staphylococcus aureus, where only the dominant tropodithietic acid (TDA) was identified. However characterisation of other metabolites is necessary as these metabolites may also serve as potent antimicrobial agents. Methods: LC-MS/MS, aided by accurate mass measurements, was employed to screen and characterise a range of metabolites produced by Pseudovibrio sp. W64 via assessment of ethyl acetate fractions generated from bacterial cultures. Results: Thirteen metabolites unique to the bacterial culture were detected and their chemical structures were assigned by tandem mass spectrometry and accurate mass measurement. Among the thirteen metabolites, a methyl ester of TDA, a number of cholic acid derivatives, and amino diols and triols were characterised. Conclusions: Pseudovibrio sp. W64 produces methylated TDA in addition to TDA, and metabolises lipids and amino acids in the cell-culture medium. To the best of our knowledge, this is the first report of methylated TDA, cholic acid and its various analogs, and sphinganine being detected in this Pseudovibrio strain. The data generated may help to better understand the biochemical processes and metabolism of bacterial strains towards discovery of antimicrobial agents from marine sources. en
dc.description.sponsorship Department of Agriculture, Food and the Marine (FIRM 11/F/009) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Inc. en
dc.rights © 2018, John Wiley & Sons Inc. This is the peer reviewed version of the following article: Choudhary, A., Naughton, L. M., Dobson, A. D.W. and Rai, D. K. (2018) 'HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters', Rapid Communications in Mass Spectrometry. doi:10.1002/rcm.8226, which has been published in final form at https://doi.org/10.1002/rcm.8226. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. en
dc.subject Pseudovibrio sp. en
dc.subject Tropodithietic acid en
dc.subject Cholic acid en
dc.subject Sphinganine en
dc.subject HPLC-MS/MS en
dc.title HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Alan Dobson, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: a.dobson@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2019-07-03
dc.date.updated 2018-07-11T10:15:57Z
dc.description.version Accepted Version en
dc.internal.rssid 444954995
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Rapid Communications in Mass Spectrometry en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress A.Dobson@ucc.ie en
dc.internal.bibliocheck In press. Check for vol. / issue / page numbers. Amend citation as necessary.


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