Position-dependent termination and widespread obligatory frameshifting in Euplotes translation

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dc.contributor.author Lobanov, Alexei V.
dc.contributor.author Heaphy, Stephen M.
dc.contributor.author Turanov, Anton A.
dc.contributor.author Gerashchenko, Maxim V.
dc.contributor.author Pucciarelli, Sandra
dc.contributor.author Devaraj, Raghul R.
dc.contributor.author Xie, Fang
dc.contributor.author Petyuk, Vladislav A.
dc.contributor.author Smith, Richard D.
dc.contributor.author Klobutcher, Lawrence A.
dc.contributor.author Atkins, John F.
dc.contributor.author Miceli, Cristina
dc.contributor.author Hatfield, Dolph L.
dc.contributor.author Baranov, Pavel V.
dc.contributor.author Gladyshev, Vadim N.
dc.date.accessioned 2018-07-30T10:30:30Z
dc.date.available 2018-07-30T10:30:30Z
dc.date.issued 2017
dc.identifier.citation Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural and Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330 en
dc.identifier.volume 24
dc.identifier.issued 1
dc.identifier.startpage 61
dc.identifier.endpage 68
dc.identifier.issn 1545-9993
dc.identifier.uri http://hdl.handle.net/10468/6518
dc.identifier.doi 10.1038/nsmb.3330
dc.description.abstract The ribosome can change its reading frame during translation in a process known as programmed ribosomal frameshifting. These rare events are supported by complex mRNA signals. However, we found that the ciliates Euplotes crassus and Euplotes focardii exhibit widespread frameshifting at stop codons. 47 different codons preceding stop signals resulted in either +1 or +2 frameshifts, and +1 frameshifting at AAA was the most frequent. The frameshifts showed unusual plasticity and rapid evolution, and had little influence on translation rates. The proximity of a stop codon to the 3' mRNA end, rather than its occurrence or sequence context, appeared to designate termination. Thus, a 'stop codon' is not a sufficient signal for translation termination, and the default function of stop codons in Euplotes is frameshifting, whereas termination is specific to certain mRNA positions and probably requires additional factors. en
dc.description.sponsorship National Institutes of Health (GM061603, GM065402, GM103493); Wellcome Trust (094423); Pacific Northwest National Laboratory/U.S. Department of Energy (Environmental Molecular Science Laboratory); U.S. Department of Energy (DE-AC05-76RLO-1830); Ministero dell’Istruzione, dell’Università e della Ricerca (Programma nazionale di Ricerche in Antartide); European Cooperation in Science and Technology (COST action (BM1102) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Nature Publishing Group en
dc.relation.uri https://www.nature.com/articles/nsmb.3330
dc.rights © 2017, Nature America, Inc., part of Springer Nature. All rights reserved. This is the peer reviewed version of the following article: Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural &Amp; Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330, which has been published in final form at https://doi.org/10.1038/nsmb.3330. en
dc.subject Ciliate en
dc.subject DNA en
dc.subject Slippage en
dc.subject Scale en
dc.subject Yeast en
dc.subject Gene en
dc.subject mRNA en
dc.title Position-dependent termination and widespread obligatory frameshifting in Euplotes translation en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Pavel V. Baranov, Biochemistry & Cell Biology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: P.Baranov@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Accepted Version en
dc.contributor.funder European Cooperation in Science and Technology
dc.contributor.funder Ministero dell’Istruzione, dell’Università e della Ricerca
dc.contributor.funder U.S. Department of Energy
dc.contributor.funder Pacific Northwest National Laboratory
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Wellcome Trust
dc.contributor.funder National Institutes of Health
dc.description.status Peer reviewed en
dc.identifier.journaltitle Nature Structural and Molecular Biology en
dc.internal.IRISemailaddress p.baranov@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IA/1335/IE/Development of computational resources for the analysis of Genome Wide Information on Protein Synthesis (GWIPS)./


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