Position-dependent termination and widespread obligatory frameshifting in Euplotes translation
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Date
2017
Authors
Lobanov, Alexei V.
Heaphy, Stephen M.
Turanov, Anton A.
Gerashchenko, Maxim V.
Pucciarelli, Sandra
Devaraj, Raghul R.
Xie, Fang
Petyuk, Vladislav A.
Smith, Richard D.
Klobutcher, Lawrence A.
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Journal ISSN
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Publisher
Nature Publishing Group
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Abstract
The ribosome can change its reading frame during translation in a process known as programmed ribosomal frameshifting. These rare events are supported by complex mRNA signals. However, we found that the ciliates Euplotes crassus and Euplotes focardii exhibit widespread frameshifting at stop codons. 47 different codons preceding stop signals resulted in either +1 or +2 frameshifts, and +1 frameshifting at AAA was the most frequent. The frameshifts showed unusual plasticity and rapid evolution, and had little influence on translation rates. The proximity of a stop codon to the 3' mRNA end, rather than its occurrence or sequence context, appeared to designate termination. Thus, a 'stop codon' is not a sufficient signal for translation termination, and the default function of stop codons in Euplotes is frameshifting, whereas termination is specific to certain mRNA positions and probably requires additional factors.
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Keywords
Ciliate , DNA , Slippage , Scale , Yeast , Gene , mRNA
Citation
Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural and Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330
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© 2017, Nature America, Inc., part of Springer Nature. All rights reserved. This is the peer reviewed version of the following article: Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural &Amp; Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330, which has been published in final form at https://doi.org/10.1038/nsmb.3330.