Starch based systems for the colonic delivery of bioactive peptides

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dc.contributor.advisor Brodkorb, André en
dc.contributor.advisor Rea, Mary C. en
dc.contributor.advisor Miao, Song en
dc.contributor.advisor Hill, Colin en
dc.contributor.author Gough, Ronan
dc.date.accessioned 2018-08-02T09:33:22Z
dc.date.available 2018-08-02T09:33:22Z
dc.date.issued 2018
dc.date.submitted 2018
dc.identifier.citation Gough, R. 2018. Starch based systems for the colonic delivery of bioactive peptides. PhD Thesis, University College Cork. en
dc.identifier.endpage 211 en
dc.identifier.uri http://hdl.handle.net/10468/6564
dc.description.abstract Bioactive peptides have numerous health benefits, although if taken orally they may be digested during gastrointestinal (GI) transit. Encapsulation is an established method for oral delivery of bioactives. However, many current approaches arise from pharmaceutical applications and may be unsuitable for food due to the materials used, cost and scale of production. Therefore, in this project we set out to create a simple and clean-label encapsulation system, suitable for use in the food industry, which could deliver bioactive peptides to the colon. One potential clean-label entrapment material is resistant starch, which is the portion of starch that resists digestion in the upper GI tract but can be digested by bacteria in the colon. As a model bioactive peptide, the well characterised antimicrobial peptide nisin was used; this peptide is normally digested during GI transit. To prepare the nisin a simple purification process was developed, which produced a powder containing ∼33% nisin from a nisin producing culture and also enriched a commercial nisin preparation over 30-fold to a purity of ∼58%. The digestion of nisin was characterised (in vitro) and 6 nisin fragments (4 of which are bioactive) were identified in the digestion products; it was also observed that nisin formed a complex with bile salts that effected its digestion. Nisin was entrapped in starch through multiple approaches based on spray coating, co-spray drying and gel entrapment. A simple approach based on gel entrapment was the most successful and it was shown in vitro to be capable of protecting a portion of the entrapped nisin during transit in the upper GI tract. Using a murine model, it was determined in vivo that a nisin entrapped in starch gel diet significantly (p < 0.001, n = 10) affected the relative abundance of 3 times as many genera from the lower GI tract than a control nisin in starch diet, despite the mice consuming 3-fold less nisin than the control diet. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2018, Ronan Gough. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Colonic delivery en
dc.subject Bioactive peptides en
dc.subject Nisin en
dc.subject Starch en
dc.title Starch based systems for the colonic delivery of bioactive peptides en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD en
dc.internal.availability Full text available en
dc.check.info Not applicable en
dc.description.version Accepted Version
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Teagasc en
dc.description.status Not peer reviewed en
dc.internal.school Microbiology en
dc.check.type No Embargo Required
dc.check.reason Not applicable en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Embargo not applicable (If you have not submitted an e-thesis or do not want to request an embargo) en
ucc.workflow.supervisor c.hill@ucc.ie
dc.internal.conferring Autumn 2018 en
dc.relation.project Department of Agriculture, Food and the Marine (grant number 10/RD/TMFRC/701); Teagasc (Walsh Fellowship Scheme, grant number 2012221) en


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