Further elucidation of galactose utilization in Lactococcus lactis MG1363
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Date
2018
Authors
Solopova, Ana
Bachman, Herwig
Teusink, Bas
Kok, Jan
Kuipers, Oscar P.
Journal Title
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Publisher
Frontiers Media S.A.
Published Version
Abstract
Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP-dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.
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Keywords
Galactose transporter , PTS , PEP-transferase system , Lactococcus lactis , GalP
Citation
Solopova, A., Bachmann, H., Teusink, B., Kok, J. and Kuipers, O. P. (2018) 'Further elucidation of galactose utilization in Lactococcus lactis MG1363', Frontiers in Microbiology, 9, 1803 (9pp). doi: 10.3389/fmicb.2018.01803