Further elucidation of galactose utilization in Lactococcus lactis MG1363

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Solopova, Ana
Bachman, Herwig
Teusink, Bas
Kok, Jan
Kuipers, Oscar P.
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Frontiers Media S.A.
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Since the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP-dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.
Galactose transporter , PTS , PEP-transferase system , Lactococcus lactis , GalP
Solopova, A., Bachmann, H., Teusink, B., Kok, J. and Kuipers, O. P. (2018) 'Further elucidation of galactose utilization in Lactococcus lactis MG1363', Frontiers in Microbiology, 9, 1803 (9pp). doi: 10.3389/fmicb.2018.01803