Reincarnation of bacteriocins From the Lactobacillus pangenomic graveyard

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dc.contributor.author Collins, Fergus W. J.
dc.contributor.author Mesa-Pereira, Beatriz
dc.contributor.author O'Connor, Paula M.
dc.contributor.author Rea, Mary C.
dc.contributor.author Hill, Colin
dc.contributor.author Ross, R. Paul
dc.date.accessioned 2018-08-29T15:47:27Z
dc.date.available 2018-08-29T15:47:27Z
dc.date.issued 2018
dc.identifier.citation Collins, F. W. J., Mesa-Pereira, B., O'Connor, P. M., Rea, M. C., Hill, C. and Ross, R. P. (2018) 'Reincarnation of Bacteriocins From the Lactobacillus Pangenomic Graveyard', Frontiers in Microbiology, 9, 1298 (9pp). doi: 10.3389/fmicb.2018.01298 en
dc.identifier.volume 9
dc.identifier.startpage 1
dc.identifier.endpage 9
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/6675
dc.identifier.doi 10.3389/fmicb.2018.01298
dc.description.abstract Bacteria commonly produce narrow spectrum bacteriocins as a means of inhibiting closely related species competing for similar resources in an environment. The increasing availability of genomic data means that it is becoming easier to identify bacteriocins encoded within genomes. Often, however, the presence of bacteriocin genes in a strain does not always translate into biological antimicrobial activity. For example, when analysing the Lactobacillus pangenome we identified strains encoding ten pediocin-like bacteriocin structural genes which failed to display inhibitory activity. Nine of these bacteriocins were novel whilst one was identified as the previously characterized bacteriocin "penocin A." The composition of these bacteriocin operons varied between strains, often with key componentsmissing which are required for bacteriocin production, such as dedicated bacteriocin transporters and accessory proteins. In an effort to functionally express these bacteriocins, the structural genes for the ten pediocin homologs were cloned alongside the dedicated pediocin PA-1 transporter in both Escherichia coli and Lactobacillus paracasei heterologous hosts. Each bacteriocin was cloned with its native leader sequence and as a fusion protein with the pediocin PA-1 leader sequence. Several of these bacteriocins displayed a broader spectrum of inhibition than the original pediocin PA-1. We show how potentially valuable bacteriocins can easily be "reincarnated" from in silico data and produced in vitro despite often lacking the necessary accompanying machinery. Moreover, the study demonstrates how genomic datasets such as the Lactobacilus pangenome harbor a potential "arsenal" of antimicrobial activity with the possibility of being activated when expressed in more genetically amenable hosts. en
dc.description.sponsorship Science Foundation Ireland (SFI/12/RC/227) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fmicb.2018.01298/full
dc.rights © 2018, Collins, Mesa Pereira, O'Connor, Rea, Hill and Ross. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Bacteriocins en
dc.subject Pediocin en
dc.subject Heterologous expression en
dc.subject Escherichia coli en
dc.subject Lactobacillus en
dc.title Reincarnation of bacteriocins From the Lactobacillus pangenomic graveyard en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul Ross, College Of Sefs Office, University College Cork, Cork, Ireland. +353-21-490-3000 Email: p.ross@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.internal.IRISemailaddress p.ross@ucc.ie en
dc.identifier.articleid 1298


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© 2018, Collins, Mesa Pereira, O'Connor, Rea, Hill and Ross. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2018, Collins, Mesa Pereira, O'Connor, Rea, Hill and Ross. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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