Assessment of charged AuNPs: from synthesis to innate immune recognition

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dc.contributor.author Rahme, Kamil
dc.contributor.author Minassian, G.
dc.contributor.author Sarkis, M.
dc.contributor.author Nakhl, M.
dc.contributor.author El Hage, Roland
dc.contributor.author Souaid, E.
dc.contributor.author Holmes, Justin D.
dc.contributor.author Ghanem, E.
dc.date.accessioned 2018-09-05T15:40:21Z
dc.date.available 2018-09-05T15:40:21Z
dc.date.issued 2018-09
dc.identifier.citation Rahme, K., Minassian, G., Sarkis, M., Nakhl, M., El Hage, R., Souaid, E., Holmes, J. D. and Ghanem, E. (2018) 'Assessment of Charged AuNPs: From Synthesis to Innate Immune Recognition', Journal of Nanomaterials, 2018, 9301912 , (12 pp). doi:10.1155/2018/9301912 en
dc.identifier.volume 2018 en
dc.identifier.startpage 9301912-1 en
dc.identifier.endpage 9301912-12 en
dc.identifier.issn 1687-4110
dc.identifier.issn 1687-4129
dc.identifier.uri http://hdl.handle.net/10468/6716
dc.identifier.doi 10.1155/2018/9301912
dc.description.abstract Gold nanoparticle (AuNP) physicochemical characteristics, mainly size and charge, modulate their biodistribution, cytotoxicity, and immunorecognition as reported from in vitro and in vivo studies. While data from in vitro studies could be biased by several factors including activation of cells upon isolation and lack of sera proteins in the microenvironment of primary generated cell lines, in vivo studies are costly and time-consuming and require ethics consideration. In this study, we developed a simple and novel in vivo-like method to test for NP immunorecognition from freshly withdrawn human blood samples. AuNPs with a size range of 30 ± 5 nm coated with cationic poly(L-lysine) (PLL) dendrigraft and slightly negative poly(vinyl alcohol) (PVA) were synthesized in water. PLL-capped AuNPs were further coated with poly(ethylene glycol) (PEG) to obtain nearly neutrally charged PEG-AuNPs. Physicochemical properties were determined using zeta potential measurements, UV-Vis spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM). Gel electrophoretic separation, zeta potential, and DLS were also used to characterize our NPs after human blood plasma treatment. PLL-AuNPs showed similar variation in charge and binding affinity to plasma proteins in comparison with PVA-AuNPs. However, PLL-AuNPs.protein complexes revealed a drastic change in size compared to the other tested particles. Results obtained from the neutrophil function test and pyridine formazan extraction revealed the highest activation level of neutrophils (~70%) by 50 μg/mL of PLL-AuNPs compared to a null induction by PEG- and PVA-AuNPs. This observation was further verified by flow cytometry analysis of polymorphonuclear cell size variation in the presence of coated AuNPs. Overall, our in vivo-like method, to test for NP immunorecognition, proved to be reliable and effective. Finally, our data supports the use of PEG-AuNPs as promising vehicles for drug delivery, as they exhibit minimal protein adsorption affinity and insignificant charge and size variation once introduced in whole blood. en
dc.description.sponsorship National Council for Scientific Research, Lebanon (CNRS-L-GRP2015) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Hindawi Publishing en
dc.relation.uri https://www.hindawi.com/journals/jnm/2018/9301912/
dc.rights © 2018 K. Rahme et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Gold nanoparticles en
dc.subject Gold en
dc.subject Nanoparticles en
dc.subject Engineered nanoparticles en
dc.subject Ligands en
dc.subject Drug delivery en
dc.subject Biodistribution en
dc.subject Immune evasion en
dc.title Assessment of charged AuNPs: from synthesis to innate immune recognition en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Justin D. Holmes, Chemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.holmes@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2018-09-05T12:18:26Z
dc.description.version Published Version en
dc.internal.rssid 452301831
dc.contributor.funder National Council for Scientific Research, Lebanon en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Nanomaterials en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress j.holmes@ucc.ie en


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© 2018 K. Rahme et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © 2018 K. Rahme et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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