An ex-vivo model to determine dental pulp responses to heat and light-curing of dental restorative materials

Show simple item record

dc.contributor.author Lynch, Christopher D.
dc.contributor.author Roberts, Jessica L.
dc.contributor.author Al-Shehri, Ali
dc.contributor.author Milward, Paul J.
dc.contributor.author Sloan, Alastair J.
dc.date.accessioned 2018-09-11T10:41:30Z
dc.date.available 2018-09-11T10:41:30Z
dc.date.issued 2018-08-31
dc.identifier.citation Lynch, C. D., Roberts, J. L., Al-Shehri, A., Milward, P. J. and Sloan, A. J. (2018) 'An ex-vivo model to determine dental pulp responses to heat and light-curing of dental restorative materials', Journal of Dentistry. doi:10.1016/j.jdent.2018.08.014 en
dc.identifier.issn 0300-5712
dc.identifier.uri http://hdl.handle.net/10468/6746
dc.identifier.doi 10.1016/j.jdent.2018.08.014
dc.description.abstract Aim: Based on histological studies from the 1960s, it is recommended that dental pulp temperature increases should not exceed 5.5 °C. However, no contemporary reliable models exist to explore the effects of heat on living dental pulp. The aim of this project was to develop a clinically valid model for studying temperature increases caused by three commonly-used light curing units (LCUs). Methods: Temperature increases caused by LCUs at varying exposure times and via various thicknesses of dentine were recorded using traditional approaches and an ex-vivo tooth slice model. Histomorphometric and immunohistochemical (IL-1β, HSP70, caspase-3) analysis was performed of the tooth slice model following varying exposure and culture times. Results: Reduced dentine thickness and increased exposure time led to increases in temperature. Whilst the majority of temperature increases recorded using the traditional approach (53 of 60) were greater than the recommended 5.5 °C, 52 of the 60 reference points recorded using the ex-vivo tooth slice model resulted in temperature increases of less than 5.5 °C. Temperature increases of 5.5 °C or more that are prolonged for 40 s caused an immediate decrease in cell number. IL-1β was not detected in any samples, while HSP70 was detectable immediately after exposure to a temperature increase of 6 °C or more. Higher levels of HSP70 were detected after 24 h culture in tooth slices that experienced a temperature increase of 7.5 °C or more. Low levels of caspase-3 were detected in tooth slices exposed to temperature increase of 7.5 °C or more. Conclusion: Experimental arrangements for assessing LCU performance that measure temperature increases using a thermocouple device on a laboratory bench should no longer be used. Future studies in this area should include replication of the clinical environment using greater sophistication, such as the use of an ex-vivo tooth slice model as described here. Temperature increases of 5.5 °C or more for 40 s caused an immediate decrease in cell number, which supports previous findings. However, complex interactions at an immunohistochemical level suggest that while temperature increases of 5 °C or less are ideal, there may be some cell damage between 5 – 7 °C which might not result in pulpal death. Further investigations are indicated. en
dc.description.sponsorship British Endodontic Society (Research Award)
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier Ltd. en
dc.rights © 2018, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Dental pulp en
dc.subject Tissue engineering en
dc.subject Root canal en
dc.subject Heat en
dc.subject Light curing en
dc.subject Composite en
dc.title An ex-vivo model to determine dental pulp responses to heat and light-curing of dental restorative materials en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Christopher Lynch, Restorative Dentistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: chris.lynch@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2019-08-31
dc.date.updated 2018-09-07T10:52:16Z
dc.description.version Accepted Version en
dc.internal.rssid 452555192
dc.contributor.funder Cardiff University en
dc.contributor.funder British Endodontic Society
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Dentistry en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress chris.lynch@ucc.ie en
dc.internal.bibliocheck In press. Check for vol. / issue / page numbers. Amend citation as necessary.


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

© 2018, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2018, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement