Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria

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dc.contributor.author Hayes, Stephen
dc.contributor.author Vincentelli, Renaud
dc.contributor.author Mahony, Jennifer
dc.contributor.author Nauta, Arjen
dc.contributor.author Ramond, Laurie
dc.contributor.author Lugli, Gabriele A.
dc.contributor.author Ventura, Marco
dc.contributor.author van Sinderen, Douwe
dc.contributor.author Cambillau, Christian
dc.date.accessioned 2018-09-20T09:10:29Z
dc.date.available 2018-09-20T09:10:29Z
dc.date.issued 2018-09-11
dc.identifier.citation Hayes, S., Vincentelli, R., Mahony, J., Nauta, A., Ramond, L., Lugli, G. A., Ventura, M., van Sinderen, D. and Cambillau, C. (2018) 'Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria', Molecular Microbiology. doi:10.1111/mmi.14124 en
dc.identifier.issn 0950-382X
dc.identifier.issn 1365-2958
dc.identifier.uri http://hdl.handle.net/10468/6822
dc.identifier.doi 10.1111/mmi.14124
dc.description.abstract With increasing numbers of 3D structures of bacteriophage components, combined with powerful in silico predictive tools, it has become possible to decipher the structural assembly and associated functionality of phage adhesion devices. Recently, decorations have been reported in the tail and neck passage structures of members of the so‐called 936 group of lactococcal siphophages. In the current report, using bioinformatic analysis we identified a conserved carbohydrate binding module (CBM) among many of the virion baseplate Dit components, in addition to the CBM present in the ‘classical’ receptor binding proteins (RBPs). We observed that, within these so‐called ‘evolved’ Dit proteins, the identified CBMs have structurally conserved folds, yet can be grouped into four distinct classes. We expressed such modules in fusion with GFP, and demonstrated their binding capability to their specific host using fluorescent binding assays with confocal microscopy. We detected evolved Dits in several phages infecting various Gram‐positive bacterial species, including mycobacteria. The omnipresence of CBM domains in Siphophages indicates their auxiliary role in infection, as they can assist in the specific recognition of and attachment to their host, thus ensuring a highly efficient and specific phage‐host adhesion process as a prelude to DNA injection. en
dc.description.sponsorship Irish Research Council (Industry Partnership Studentship); French Infrastructure for Integrated Structural Biology (ANR-10-INSB-05-01) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Inc. en
dc.rights © 2018, John Wiley & Sons Inc. This is the peer reviewed version of the following article: Hayes, S., Vincentelli, R., Mahony, J., Nauta, A., Ramond, L., Lugli, G. A., Ventura, M., van Sinderen, D. and Cambillau, C. (2018) 'Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria', Molecular Microbiology. doi:10.1111/mmi.14124, which has been published in final form at https://doi.org/10.1111/mmi.14124. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. en
dc.subject Bacteriophage en
dc.subject Lactococcus lactis en
dc.subject Receptor-binding protein en
dc.subject Distal tail protein en
dc.subject Carbohydrate binding module en
dc.subject Phage-host interactions en
dc.title Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2019-09-11
dc.date.updated 2018-09-20T08:59:52Z
dc.description.version Accepted Version en
dc.internal.rssid 454454647
dc.contributor.funder Irish Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder French Infrastructure for Integrated Structural Biology en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Molecular Microbiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.internal.bibliocheck In press. Check for vol. / issue / page numbers. Amend citation as necessary.
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/ en


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