Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content

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dc.contributor.author Oliveira Jr., Getulio P.
dc.contributor.author Porto, William F.
dc.contributor.author Palu, Cintia C.
dc.contributor.author Pereira, Lydyane M.
dc.contributor.author Petriz, Bernardo
dc.contributor.author Almeida, Jeeser A.
dc.contributor.author Viana, Juliane
dc.contributor.author Filho, Nezio N. A.
dc.contributor.author Franco, Octavio L.
dc.contributor.author Pereira, Rinaldo W.
dc.date.accessioned 2018-09-24T12:36:55Z
dc.date.available 2018-09-24T12:36:55Z
dc.date.issued 2018
dc.identifier.citation Oliveira, G. P., Porto, W. F., Palu, C. C., Pereira, L. M., Petriz, B., Almeida, J. A., Viana, J., Filho, N. N. A., Franco, O. L. and Pereira, R. W. (2018) 'Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content', Frontiers in Physiology, 9, 532 (11pp). doi: 10.3389/fphys.2018.00532 en
dc.identifier.volume 9
dc.identifier.startpage 1
dc.identifier.endpage 11
dc.identifier.issn 1664-042X
dc.identifier.uri http://hdl.handle.net/10468/6868
dc.identifier.doi 10.3389/fphys.2018.00532
dc.description.abstract Physical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other organs communicate to translate exercise into health. Low, moderate and high intensity treadmill protocols were applied to rat groups, aiming to investigate the impact of exercise on serum EVs and their associated small RNA molecules. Transmission electron microscopy, resistive pulse sensing, and western blotting were used to investigate EVs morphology, size distribution, concentration and EVs marker proteins. Small RNA libraries from EVs RNA were sequenced. Exercise did not change EVs size, while increased EVs concentration. Twelve miRNAs were found differentially expressed after exercise: rno-miR-128-3p, 1033p, 330-5p, 148a-3p, 191a-5p, 10b-5p, 93-5p, 25-3p, 142-5p, 3068-3p, 142-3p, and 410-3p. No piRNA was found differentially expressed, and one tRNA, trna8336, was found down-regulated after exercise. The differentially expressed miRNAs were predicted to target genes involved in the MAPK pathway. A single bout of exercise impacts EVs and their small RNA load, reinforcing the need for a more detailed investigation into EVs and their load as mediators of health-promoting exercise. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media SA en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fphys.2018.00532/full
dc.rights © 2018, Oliveira, Porto, Palu, Pereira, Petriz, Almeida, Viana, Filho, Franco and Pereira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Extracellular vesicles en
dc.subject Small RNA en
dc.subject Aerobic exercise en
dc.subject Nextgene en
dc.subject Edger en
dc.title Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Cintia Palu, Biochemistry & Cell Biology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: cintia.palu@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Irish Research Council
dc.contributor.funder Universidade Católica de Brasília
dc.contributor.funder Fundação de Apoio à Pesquisa do Distrito Federal
dc.contributor.funder Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.contributor.funder Ministério da Educação
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Physiology en
dc.internal.IRISemailaddress cintia.palu@ucc.ie en
dc.identifier.articleid 532


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© 2018, Oliveira, Porto, Palu, Pereira, Petriz, Almeida, Viana, Filho, Franco and Pereira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2018, Oliveira, Porto, Palu, Pereira, Petriz, Almeida, Viana, Filho, Franco and Pereira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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