A diverse range of human gut bacteria have the potential to metabolize the dietary component gallic acid

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dc.contributor.author Esteban-Torres, María
dc.contributor.author Santamaría, Laura
dc.contributor.author Cabrera-Rubio, Raul
dc.contributor.author Plaza-Vinuesa, Laura
dc.contributor.author Crispie, Fiona
dc.contributor.author de las Rivas, Blanca
dc.contributor.author Cotter, Paul D.
dc.contributor.author Muñoz, Rosario
dc.date.accessioned 2018-11-07T11:34:21Z
dc.date.available 2018-11-07T11:34:21Z
dc.date.issued 2018-07-27
dc.identifier.citation Esteban-Torres, M., Santamaría, L., Cabrera-Rubio, R., Plaza-Vinuesa, L., Crispie, F., de las Rivas, B., Cotter, P. and Muñoz, R. (2018) 'A diverse range of human gut bacteria have the potential to metabolize the dietary component gallic acid', Applied and Environmental Microbiology, 84(19), e01558-18 (12pp). doi:10.1128/aem.01558-18 en
dc.identifier.volume 84 en
dc.identifier.issued 19 en
dc.identifier.issn 0099-2240
dc.identifier.issn 1098-5336
dc.identifier.uri http://hdl.handle.net/10468/7068
dc.identifier.doi 10.1128/aem.01558-18
dc.description.abstract The human gut microbiota contains a broad variety of bacteria that possess functional genes, with resultant metabolites that affect human physiology and therefore health. Dietary gallates are phenolic components that are present in many foods and beverages and are regarded as having health-promoting attributes. However, the potential for metabolism of these phenolic compounds by the human microbiota remains largely unknown. The emergence of high-throughput sequencing (HTS) technologies allows this issue to be addressed. In this study, HTS was used to assess the incidence of gallate-decarboxylating bacteria within the gut microbiota of healthy individuals for whom bacterial diversity was previously determined to be high. This process was facilitated by the design and application of degenerate PCR primers to amplify a region encoding the catalytic C subunit of gallate decarboxylase (LpdC) from total metagenomic DNA extracted from human fecal samples. HTS resulted in the generation of a total of 3,261,967 sequence reads and revealed that the primary gallate-decarboxylating microbial phyla in the intestinal microbiota were Firmicutes (74.6%), Proteobacteria (17.6%), and Actinobacteria (7.8%). These reads corresponded to 53 genera, i.e., 47% of the bacterial genera detected previously in these samples. Among these genera, Anaerostipes and Klebsiella accounted for the majority of reads (40%). The usefulness of the HTS-lpdC method was demonstrated by the production of pyrogallol from gallic acid, as expected for functional gallate decarboxylases, among representative strains belonging to species identified in the human gut microbiota by this method. Importance: Despite the increasing wealth of sequencing data, the health contributions of many bacteria found in the human gut microbiota have yet to be elucidated. This study applies a novel experimental approach to predict the ability of gut microbes to carry out a specific metabolic activity, i.e., gallate metabolism. The study showed that, while gallate-decarboxylating bacteria represented 47% of the bacterial genera detected previously in the same human fecal samples, no gallate decarboxylase homologs were identified from representatives of Bacteroidetes. The presence of functional gallate decarboxylases was demonstrated in representative Proteobacteria and Firmicutes strains from the human microbiota, an observation that could be of considerable relevance to the in vivo production of pyrogallol, a physiologically important bioactive compound. en
dc.description.sponsorship Ministerio de Economía y Competitividad (FPI Fellowship; Grant AGL2014-52911-R (AEI/FEDER, UE)); Irish Research Council (Postdoctoral Grant GOIPD/2017/1302) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.uri https://aem.asm.org/content/aem/84/19/e01558-18.full.pdf
dc.rights © 2018, American Society for Microbiology. All rights reserved. en
dc.subject HTS en
dc.subject Antioxidant en
dc.subject Human intestinal tract en
dc.subject Microbiota en
dc.subject Phenolic compounds en
dc.subject Pyrogallol en
dc.title A diverse range of human gut bacteria have the potential to metabolize the dietary component gallic acid en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Marí­a Del Mar Esteban-Torres, Alimentary Pharmabotic Centre (APC), University College Cork, Cork, Ireland. +353-21-490-3000 Email: maria.estebantorres@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 6 months after publication by request of the publisher. en
dc.check.date 2019-01-27
dc.date.updated 2018-11-06T12:27:49Z
dc.description.version Published Version en
dc.internal.rssid 460845084
dc.contributor.funder Irish Research Council en
dc.contributor.funder Ministerio de Economía y Competitividad en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Applied and Environmental Microbiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress maria.estebantorres@ucc.ie en

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