Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes
Loading...
Files
Accepted Version
Date
2018-12-06
Authors
Dalfen, Irene
Dmitriev, Ruslan I.
Holst, Gerhard
Klimant, Ingo
Borisov, Sergey M.
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Published Version
Abstract
Novel fluorescent diazaoxatriangulenium (DAOTA) pH indicators for lifetime-based self-referenced pH sensing are reported. The DAOTA dyes were decorated with phenolic receptor groups inducing fluorescence quenching via photoinduced electron transfer mechanism. Electron-withdrawing chlorine substituents ensure response in the most relevant pH range (apparent pK'a values ~5 and 7.5 for the p,p-dichlorophenol- and the p-chlorophenol-substituted dyes, respectively). The dyes feature long fluorescence lifetime (17-20 ns), high quantum yield (~60%) and high photostability. Planar optodes are prepared upon immobilization of the dyes into polyurethane hydrogel D4. Apart from the response in the fluorescence intensity, the optodes show pH-dependent lifetime behaviour which makes them suitable for studying 2D pH distribution with help of fluorescence lifetime imaging technique. The lifetime response is particularly pronounced for the sensors with high dye concentration (0.5-1% wt. in respect to the polymer) and is attributed to efficient homo-FRET mechanism.
Description
Keywords
Decay time , FRET , pH sensor , Triangulenium , Photoinduced electron transfer , Frequency Domain FLIM
Citation
Dalfen, I., Dmitriev, R. I., Holst, G., Klimant, I.and Borisov, S. M. (2018) 'Background-free fluorescence decay time sensing and imaging of pH with highly photostable diazaoxotriangulenium dyes', Analytical Chemistry. doi:10.1021/acs.analchem.8b02534
Link to publisher’s version
Copyright
© 2018, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry after technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.analchem.8b02534