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Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages
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Date
2018-12-31
Authors
O'Sullivan, Siobhán M.
O'Callaghan, Yvonne C.
O'Keeffe, Martina B.
FitzGerald, Richard J.
O'Brien, Nora M.
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Published Version
Abstract
The in vitro bioactivity of 5 kDa ultrafiltration permeate fractions of casein hydrolysates produced using different enzymes were compared. Reverse phase ultra-performance liquid chromatography and gel permeation chromatography showed that the permeates had different physicochemical properties (molecular mass and degree of hydrolysis). The Flavourzyme® permeate had the highest activity in the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Cellular antioxidant and immunomodulatory assays showed that none of the permeates exhibited in vitro antioxidant activity, while all permeates significantly (P < 0.05) decreased interleukin-6 (IL-6) production in ConA-stimulated Jurkat T cells at 0.50% (w/v) and LPS-stimulated RAW264.7 cells at 0.05 and 0.50% (w/v). Three permeates, obtained using Flavourzyme®, Flavorpro Whey and trypsin, also significantly (P < 0.05) decreased IL-1β production at 0.05% (w/v) in RAW264.7 cells. Western blot analysis showed that all permeates significantly decreased the expression of the NF-κB subunit, p65, in RAW264.7 cells indicating that anti-inflammatory activity may be associated with this pathway.
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Keywords
Casein , Bioactivity , Protein hydrolysates , Anti-inflammatory activity , In vitro bioactivity , Casein hydrolysates
Citation
O’Sullivan, S. M., O’Callaghan, Y. C., O’Keeffe, M. B., FitzGerald, R. J. and O’Brien, N. M. (2018) 'Immunomodulatory activity of 5 kDa permeate fractions of casein hydrolysates generated using a range of enzymes in Jurkat T cells and RAW264.7 macrophages', International Dairy Journal, In Press, doi: 10.1016/j.idairyj.2018.12.005