Identification of an esterase isolated using metagenomic technology which displays an unusual substrate scope and its characterisation as an enantioselective biocatalyst

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dc.contributor.author Gavin, Declan P.
dc.contributor.author Murphy, Edel J.
dc.contributor.author Foley, Aoife M.
dc.contributor.author Castilla, Ignacio Abreu
dc.contributor.author Reen, F. Jerry
dc.contributor.author Woods, David F.
dc.contributor.author Collins, Stuart G.
dc.contributor.author O'Gara, Fergal
dc.contributor.author Maguire, Anita R.
dc.date.accessioned 2019-02-26T15:33:16Z
dc.date.available 2019-02-26T15:33:16Z
dc.date.issued 2019-02-11
dc.identifier.citation Gavin, D. P., Murphy, E. J., Foley, A. M., Abreu Castilla, I., Reen, F. J., Woods, D. F., Collins, S. G., O'Gara, F. and Maguire, A. (2019) 'Identification of an Esterase Isolated Using Metagenomic Technology which Displays an Unusual Substrate Scope and its Characterisation as an Enantioselective Biocatalyst', Advanced Synthesis & Catalysis, In Press, doi: 10.1002/adsc.201801691 en
dc.identifier.startpage 1 en
dc.identifier.endpage 12 en
dc.identifier.issn 1615-4150
dc.identifier.uri http://hdl.handle.net/10468/7543
dc.identifier.doi 10.1002/adsc.201801691
dc.description.abstract Evaluation of an esterase annotated as 26D isolated from a marine metagenomic library is described. Esterase 26D was found to have a unique substrate scope, including synthetic transformations which could not be readily effected in a synthetically useful manner using commercially available enzymes. Esterase 26D was more selective towards substrates which had larger, more sterically demanding substituents (i.e. iso‐propyl or tert‐butyl groups) on the β‐carbon, which is in contrast to previously tested commercially available enzymes which displayed a preference for substrates with sterically less demanding substituents (i.e. methyl group) at the β‐carbon. en
dc.description.sponsorship Irish Research Council (EPSPG/2016/41); Enterprise Ireland (CF-2017-0757-P; IP-2015-0390); Department of Agriculture, Food and the Marine ((FIRM 11/F009/MabS; FIRM 13/F/516); (BEAU/BIOD/01)); Irish Research Council for Science, Engineering and Technology (GOIPG/2014/647); Health Research Board and Irish Thoracic Society (MRCG-2014-6) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Wiley en
dc.relation.uri https://onlinelibrary.wiley.com/doi/abs/10.1002/adsc.201801691
dc.rights © 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: (2019), Identification of an Esterase Isolated Using Metagenomic Technology which Displays an Unusual Substrate Scope and its Characterisation as an Enantioselective Biocatalyst. Adv. Synth. Catal., Accepted Author Manuscript, which has been published in final form at https://doi.org/10.1002/adsc.201801691. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving." en
dc.subject Enantiopurity en
dc.subject Metagenomic library en
dc.subject Stereochemistry en
dc.subject Biocatalyst en
dc.subject Esterase en
dc.title Identification of an esterase isolated using metagenomic technology which displays an unusual substrate scope and its characterisation as an enantioselective biocatalyst en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Anita Maguire, Vice President For Research, University College Cork, Cork, Ireland. +353-21-490-3000 Email: a.maguire@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2020-02-11
dc.date.updated 2019-02-25T16:41:25Z
dc.description.version Accepted Version en
dc.internal.rssid 474905132
dc.contributor.funder Irish Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Seventh Framework Programme en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Food Institutional Research Measure en
dc.contributor.funder Health Research Board en
dc.contributor.funder Irish Thoracic Society en
dc.contributor.funder Cystic Fibrosis Foundation en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Advanced Synthesis & Catalysis en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress a.maguire@ucc.ie en
dc.internal.bibliocheck In Press, Feb 2019. Update citation details, vol., page numbers. en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/ en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3 en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA) - Training Award/13/TIDA/B2625 TIDA Training 2014/IE/Small molecule inhibitors of HIF-1: a new class of anti-cancer therapeutics./ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/12/TIDA/B2411/IE/Development of small molecule therapeutics for medical intervention: anti-biofilm inhibitors for the medical device sector./ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/12/TIDA/B2405/IE/Optimised detection of key biomarkers of Pseudomonas aeruginosa towards a clinical application/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/14/TIDA/2438/IE/Next Generation Antibiotics: anti-biofilm, anti-pathogenic natural bioactives from marine microorganisms./ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/15/TIDA/2977/IE/Pre-clinical testing of novel fungal biofilm blockers for the medical device sector./ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Frontiers Programme (RFP)/09/RFP/BMT2350/IE/An investigation of the molecular interactions between statins and microbial pathogens and their combined impact on the human immune response./ en


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