The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori

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dc.contributor.author Douillard, François P.
dc.contributor.author Ryan, Kieran A.
dc.contributor.author Lane, Michael C.
dc.contributor.author Caly, Delphine L.
dc.contributor.author Moore, Stanley A.
dc.contributor.author Penn, Charles W.
dc.contributor.author Hinds, Jason
dc.contributor.author O'Toole, Paul W.
dc.date.accessioned 2012-11-12T13:25:14Z
dc.date.available 2012-11-12T13:25:14Z
dc.date.copyright 2010
dc.date.issued 2010-04-08
dc.identifier.citation Douillard, F.P., K. A. Ryan, M. C. Lane, D.L. Caly, S. A. Moore, C. W. Penn, J. Hinds, and P. W. O’Toole. (2010). The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori. BMC Microbiology, 10:106 doi:10.1186/1471-2180-10-106 en
dc.identifier.issued 10 en
dc.identifier.startpage 106 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/757
dc.identifier.doi 10.1186/1471-2180-10-106
dc.description.abstract Background: Helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in H. pylori has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In Salmonella, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament. Results: Ablation of the HP0256 gene in H. pylori significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells. Conclusions: We conclude that HP0256 is not a functional counterpart of FliJ in H. pylori. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion. en
dc.description.sponsorship Science Foundation Ireland (CSET); Science Foundation Ireland (Research Frontiers Programme); Natural Sciences and Engineering Research Council of Canada (Discovery Grant RGPIN262138-05) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central en
dc.relation.uri http://www.biomedcentral.com/1471-2180/10/106
dc.rights © 2010 Douillard et al; licensee BioMed Central Ltd. en
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en
dc.subject Helicobacter pylori en
dc.subject HP0256 en
dc.subject FliJ en
dc.subject Gastritis en
dc.subject Ulcer en
dc.subject Salmonella en
dc.subject Cell envelope architecture en
dc.title The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://research.ucc.ie/profiles/D010/pwotoole en
dc.internal.authorcontactother Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Ireland. Email: pwotoole@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 43334297
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Natural Sciences and Engineering Research Council of Canada en
dc.contributor.funder Wellcome Trust, United Kingdom en
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en


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© 2010 Douillard et al; licensee BioMed Central Ltd. Except where otherwise noted, this item's license is described as © 2010 Douillard et al; licensee BioMed Central Ltd.
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