Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

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dc.contributor.author O'Toole, Paul W.
dc.contributor.author Snelling, William J.
dc.contributor.author Canchaya, Carlos
dc.contributor.author Forde, Brian M.
dc.contributor.author Hardie, Kim R.
dc.contributor.author Josenhans, Christine
dc.contributor.author Graham, Robert L.J.
dc.contributor.author McMullan, Geoff
dc.contributor.author Parkhill, Julian
dc.contributor.author Belda, Eugenio
dc.contributor.author Bentley, Stephen D.
dc.date.accessioned 2012-11-14T14:07:06Z
dc.date.available 2012-11-14T14:07:06Z
dc.date.copyright 2010
dc.date.issued 2010-03-10
dc.identifier.citation O'Toole, P. W., W. J. Snelling, et al. (2010). "Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen." BMC Genomics 11: 164. doi: 10.1186/1471-2164-11-164 en
dc.identifier.issued 11 en
dc.identifier.startpage 164 en
dc.identifier.endpage n/a en
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10468/777
dc.identifier.doi 10.1186/1471-2164-11-164
dc.description.abstract Background: Helicobacter mustelae causes gastritis, ulcers and gastric cancer in ferrets and other mustelids. H. mustelae remains the only helicobacter other than H. pylori that causes gastric ulceration and cancer in its natural host. To improve understanding of H. mustelae pathogenesis, and the ulcerogenic and carcinogenic potential of helicobacters in general, we sequenced the H. mustelae genome, and identified 425 expressed proteins in the envelope and cytosolic proteome. Results: The H. mustelae genome lacks orthologs of major H. pylori virulence factors including CagA, VacA, BabA, SabA and OipA. However, it encodes ten autotransporter surface proteins, seven of which were detected in the expressed proteome, and which, except for the Hsr protein, are of unknown function. There are 26 putative outer membrane proteins in H. mustelae, some of which are most similar to the Hof proteins of H. pylori. Although homologs of putative virulence determinants of H. pylori (NapA, plasminogen adhesin, collagenase) and Campylobacter jejuni (CiaB, Peb4a) are present in the H. mustelae genome, it also includes a distinct complement of virulence-related genes including a haemagglutinin/haemolysin protein, and a glycosyl transferase for producing blood group A/B on its lipopolysaccharide. The most highly expressed 264 proteins in the cytosolic proteome included many corresponding proteins from H. pylori, but the rank profile in H. mustelae was distinctive. Of 27 genes shown to be essential for H. pylori colonization of the gerbil, all but three had orthologs in H. mustelae, identifying a shared set of core proteins for gastric persistence. Conclusions: The determination of the genome sequence and expressed proteome of the ulcerogenic species H mustelae provides a comparative model for H. pylori to investigate bacterial gastric carcinogenesis in mammals, and to suggest ways whereby cag minus H. pylori strains might cause ulceration and cancer. The genome sequence was deposited in EMBL/GenBank/DDBJ under accession number FN555004. en
dc.description.sponsorship Science Foundation Ireland (SFI CSET); Science Foundation Ireland (PI awards); Department of Agriculture, Fisheries and Food, Ireland (FHRI Award to the Eldermet Project); European Union Program for Peace and Reconciliation (EU Technology Support for the Knowledge-Based Economy); Bundesministerium für Bildung und Forschung, Germany (ERAnet PathoGenomics) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central en
dc.relation.uri http://www.biomedcentral.com/1471-2164/11/164
dc.rights © 2010 O'Toole et al; licensee BioMed Central Ltd. en
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en
dc.subject Helicobacter mustelae en
dc.subject Ulcerogenic en
dc.subject Carcinogenic en
dc.subject Helicobacter en
dc.subject H. pylori en
dc.subject FN555004 en
dc.title Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://research.ucc.ie/profiles/D010/pwotoole en
dc.internal.authorcontactother Paul O'Toole, Department of Microbiology, & Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland. Email: pwotoole@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 43334382
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Department of Agriculture, Fisheries and Food, Ireland en
dc.contributor.funder Northern Ireland Centre of Excellence in Functional Genomics en
dc.contributor.funder European Union Program for Peace and Reconciliation en
dc.contributor.funder Bundesministerium für Bildung und Forschung, Germany de
dc.contributor.funder Wellcome Trust, United Kingdom en
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Genomics en
dc.internal.IRISemailaddress pwotoole@ucc.ie en


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© 2010 O'Toole et al; licensee BioMed Central Ltd. Except where otherwise noted, this item's license is described as © 2010 O'Toole et al; licensee BioMed Central Ltd.
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