The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis

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dc.contributor.author McDermott, Kieran
dc.contributor.author Kiernan, Miranda G.
dc.contributor.author Kiely, Patrick A.
dc.contributor.author Dunne, Colum P.
dc.contributor.author Coffey, J. Calvin
dc.contributor.author Cotter, Paul D.
dc.contributor.author Cabrera-Rubio, Raul
dc.date.accessioned 2019-05-15T05:11:02Z
dc.date.available 2019-05-15T05:11:02Z
dc.date.issued 2018-09-15
dc.identifier.citation Kiernan, M.G., Coffey, J.C., McDermott, K., Cotter, P.D., Cabrera-Rubio, R., Kiely, P.A. and Dunne, C.P., 2018. The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis. Journal of Crohn's and Colitis, 13(1), pp. 58-66. DOI: 10.1093/ecco-jcc/jjy136 en
dc.identifier.volume 13 en
dc.identifier.issued 1 en
dc.identifier.startpage 58 en
dc.identifier.endpage 66 en
dc.identifier.issn 1873-9946
dc.identifier.uri http://hdl.handle.net/10468/7905
dc.identifier.doi 10.1093/ecco-jcc/jjy136 en
dc.description.abstract Mesenteric lymph nodes are sites in which translocated bacteria incite and progress immunological responses. For this reason, understanding the microbiome of mesenteric lymph nodes in inflammatory bowel disease is important. The bacterial profile of Crohn’s disease mesenteric lymph nodes has been analysed using culture-independent methods in only one previous study. This study aimed to investigate the mesenteric lymph node microbiota from both Crohn’s disease and ulcerative colitis patients.Mesenteric lymph nodes were collected from Crohn’s disease and ulcerative colitis patients undergoing resection. Total DNA was extracted from mesenteric lymph nodes and assessed for the presence of bacterial DNA [16S]. All work was completed in a sterile environment using aseptic techniques. Samples positive for 16S DNA underwent next-generation sequencing, and the identity of bacterial phyla and species were determined.Crohn’s disease mesenteric lymph nodes had a distinctly different microbial profile to that observed in ulcerative colitis. The relative abundance of Firmicutes was greater in nodes from ulcerative colitis patients, whereas Proteobacteria were more abundant in Crohn’s disease. Although species diversity was reduced in the mesenteric lymph nodes of patients with Crohn’s disease, these lymph nodes contained greater numbers of less dominant phyla, mainly Fusobacteria.This study confirms that there are distinct differences between the Crohn’s disease and ulcerative colitis mesenteric lymph node microbiomes. Such microbial differences could aid in the diagnosis of Crohn’s disease or ulcerative colitis, particularly in cases of indeterminate colitis at time of resection, or help explain their mechanisms of development and progression. en
dc.description.sponsorship University of Limerick (Graduate Entry Medical School Strategic Research Fund) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Oxford University Press en
dc.relation.uri https://academic.oup.com/ecco-jcc/article/13/1/58/5098279
dc.rights © European Crohn’s and Colitis Organisation (ECCO) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Inflammatory bowel disease en
dc.subject Microbiome en
dc.subject Mesenteric lymph nodes en
dc.title The human mesenteric lymph node microbiome differentiates between Crohn’s disease and ulcerative colitis en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul Cotter, APC Microbiome Institute, University College Cork, Cork, Ireland. +353-21-490-3000 Email: paul.cotter@teagasc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder University of Limerick en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Crohn's and Colitis en
dc.internal.IRISemailaddress paul.cotter@teagasc.ie en


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© European Crohn’s and Colitis Organisation (ECCO) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © European Crohn’s and Colitis Organisation (ECCO) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
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