The dissemination of C10 cysteine protease genes in Bacteroides fragilis species by mobile genetic elements

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dc.contributor.author Thornton, Roibeard F.
dc.contributor.author Kagawa, Todd F.
dc.contributor.author O'Toole, Paul W.
dc.contributor.author Cooney, Jakki C.
dc.date.accessioned 2012-11-21T15:45:09Z
dc.date.available 2012-11-21T15:45:09Z
dc.date.copyright 2010
dc.date.issued 2010-04-23
dc.identifier.citation Thornton, R, T.F. Kagawa, P.W. O’Toole and J.C. Cooney. 2010. The dissemination of C10 cysteine protease genes in Bacteroides fragilis species by mobile genetic elements. BMC Microbiol. 10: 122. DOI: 10.1186/1471-2180-10-122 en
dc.identifier.issued 10 en
dc.identifier.startpage 122 en
dc.identifier.issn 1471-2180
dc.identifier.uri http://hdl.handle.net/10468/793
dc.identifier.doi 10.1186/1471-2180-10-122
dc.description.abstract Background: The C10 family of cysteine proteases includes enzymes that contribute to the virulence of bacterial pathogens, such as SpeB in Streptococcus pyogenes. The presence of homologues of cysteine protease genes in human commensal organisms has not been examined. Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. Results: Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. These four protease genes, two were directly contiguous to open reading frames predicted to encode staphostatin-like inhibitors, with which the protease genes were co-transcribed. Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. Gene annotation indicated that one of these insertions was a conjugative Tn-like element and the other was a prophage-like element, which was shown to be capable of excision. Homologues of the B. fragilis C10 protease genes were present in a panel of clinical isolates, and in DNA extracted from normal human faecal microbiota. Conclusions: This study suggests a mechanism for the evolution and dissemination of an important class of protease in major members of the normal human microbiota. en
dc.description.sponsorship Science Foundation Ireland (08/RFP/BMT1596); Science Foundation Ireland (SFI-CSET); University of Limerick (UL PhD Studentship); Government of Ireland Dept of Agriculture Fisheries and Food (DAFF FHRI) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central en
dc.relation.uri http://www.biomedcentral.com/1471-2180/10/122
dc.rights © 2010 Thornton et al; licensee BioMed Central Ltd. en
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en
dc.subject C10 en
dc.subject Cysteine Proteases en
dc.subject Streptococcus pyogenes en
dc.subject Bacteroides fragilis en
dc.subject SpeB en
dc.subject Human Microbiota en
dc.title The dissemination of C10 cysteine protease genes in Bacteroides fragilis species by mobile genetic elements en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://research.ucc.ie/profiles/D010/pwotoole en
dc.internal.authorcontactother Department of Microbiology, & Alimentary Pharmabiotic Centre, University College Cork, Ireland. Email: pwotoole@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder University of Limerick en
dc.contributor.funder Department of Agriculture, Fisheries and Food, Ireland en
dc.contributor.funder Wellcome Trust, United Kingdom en
dc.description.status Peer reviewed en
dc.identifier.journaltitle BMC Microbiology en
dc.internal.IRISemailaddress pwotoole@ucc.ie en


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© 2010 Thornton et al; licensee BioMed Central Ltd. Except where otherwise noted, this item's license is described as © 2010 Thornton et al; licensee BioMed Central Ltd.
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