Bioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cells

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dc.contributor.author Rahme, Kamil
dc.contributor.author Guo, Jianfeng
dc.contributor.author Holmes, Justin D.
dc.contributor.editor Dinesh Kumar, Lekha
dc.date.accessioned 2019-05-22T15:51:03Z
dc.date.available 2019-05-22T15:51:03Z
dc.date.issued 2019-05-17
dc.identifier.citation Rahme, K., Guo, J. and Holmes, J. D. (2019) 'Bioconjugated Gold Nanoparticles Enhance siRNA Delivery in Prostate Cancer Cells', in Dinesh Kumar, L. (ed.) RNA Interference and Cancer Therapy: Methods and Protocols. New York, NY: Springer New York, pp. 291-301. doi: 10.1007/978-1-4939-9220-1_21 en
dc.identifier.startpage 291 en
dc.identifier.endpage 301 en
dc.identifier.uri http://hdl.handle.net/10468/7970
dc.identifier.doi 10.1007/978-1-4939-9220-1_21 en
dc.description.abstract Here we describe a simple way to create a gold nanoparticle (AuNP)-based non-viral delivery system to deliver siRNA into prostate cancer cells. Therefore, positively charged polyethylenimine (PEI)-capped AuNPs were synthesized in water and further conjugated with the targeting ligand (folic acid) for folate receptors (AuNPs-PEI-FA). The AuNPs-PEI-FA could effectively complex small interfering RNA (siRNA) through electrostatic interaction. Flow cytometry displayed that AuNPs-PEI-FA could specifically deliver siRNA into LNCaP cells, a prostate cancer cell line overexpressing prostate-specific membrane antigen (PSMA) that exhibits a hydrolase enzymatic activity with a folate substrate. In contrast, internalization of siRNA into PC-3 cells, a prostate cancer cell line not expressing PSMA or folate receptors, was not achieved using AuNPs-PEI-FA.siRNA. Following endolysosomal escape, the AuNPs-PEI-FA-.siRNA formulation resulted in significant endogenous gene silencing when compared to the nontargeted formulation, suggesting the potential of AuNPs-PEI-FA for targeted delivery of therapeutic siRNAs in the treatment of prostate cancer. en
dc.description.sponsorship Irish Research Council (Government of Ireland Postdoctoral Fellowship (GOIPD/2013/150)); Department of Science and Technology of Jilin Province (Outstanding Youth Foundation from the Department of Science and Technology, Jilin Province (Project Number: 20170520046JH)); National Council for Scientific Research, Lebanon (CNRS-L-GRP2015-3538). en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Springer en
dc.relation.ispartof RNA Interference and Cancer Therapy: Methods and Protocols, Methods in Molecular Biology
dc.relation.uri https://link.springer.com/protocol/10.1007%2F978-1-4939-9220-1_21
dc.rights © 2019 Springer. This is a post-peer-review, pre-copyedit version of a book chapter published in RNA Interference and Cancer Therapy Methods and Protocols; Methods in Molecular Biology book series (MIMB, volume 1974), The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9220-1_21 en
dc.subject Gold nanoparticles en
dc.subject Targeting ligands en
dc.subject Receptor-mediated internalization en
dc.subject Non-viral siRNA delivery en
dc.subject Prostate cancer en
dc.subject Gene therapy en
dc.title Bioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cells en
dc.type Book chapter en
dc.internal.authorcontactother Justin D. Holmes, Chemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.holmes@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2020-05-17
dc.date.updated 2019-05-22T09:23:05Z
dc.description.version Accepted Version en
dc.internal.rssid 486046559
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Irish Research Council en
dc.contributor.funder Department of Science and Technology of Jilin Province en
dc.contributor.funder National Council for Scientific Research en
dc.description.status Peer reviewed en
dc.identifier.journaltitle RNA Interference and Cancer Therapy: Methods and Protocols en
dc.internal.copyrightchecked No
dc.internal.licenseacceptance Yes en
dc.internal.placepublication New York, USA en
dc.internal.IRISemailaddress j.holmes@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2278/IE/Advanced Materials and BioEngineering Research Centre (AMBER)/ en


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